Articles
GENETIC ANALYSIS AND THE SSR-BASED GENETIC LINKAGE MAP OF BLUNTED WITH BLOSSOM TRAIT IN CUCUMIS SATIVUS L.
Article number
871_9
Pages
87 – 92
Language
English
Abstract
In order to investigate the heredity of the blunt with blossom cucumbers (BBCs), the genetic analysis of BBCs traits and a genetic linkage map using simple sequence repeat (SSR) markers were constructed.
Besides an F1 population from a cross between D0462, a blossom cucumbers (BBCs) ecotype, and a normal ecotype 129, the F2 consisting of 288 individuals, and BC1 populations were used for the genetic analysis and genetic mapping.
The results indicated that heterozygous F1 had no BBCs, which indicated that the BBCs trait was recessive.
In addition, the segregation rate between BBCs species and normal species in the BC1 and F2 populations was 1:1.2 and 2:2.69, respectively, which confirmed that the BBCs trait (de) is recessive to the normal trait (De). Further more, a total of 86 SSR markers were screened for loci and polymorphism using DNA from two parents and 288 F2s of the mapping population, and only 4 primer pairs were demonstrated to produce fragments of expected size.
Of the 4 products, 3 (CWGATT01A, CSWGATT01C and CSWTAAA01) were mapped to the same linkage group by use of MAPMAKER/EXP, version 3.0b, and de was illustrated in the linkage group located between CSWGATT01C and CSWTAAA01 at 9.5 and 21.5 cM from the markers, respectively.
Besides an F1 population from a cross between D0462, a blossom cucumbers (BBCs) ecotype, and a normal ecotype 129, the F2 consisting of 288 individuals, and BC1 populations were used for the genetic analysis and genetic mapping.
The results indicated that heterozygous F1 had no BBCs, which indicated that the BBCs trait was recessive.
In addition, the segregation rate between BBCs species and normal species in the BC1 and F2 populations was 1:1.2 and 2:2.69, respectively, which confirmed that the BBCs trait (de) is recessive to the normal trait (De). Further more, a total of 86 SSR markers were screened for loci and polymorphism using DNA from two parents and 288 F2s of the mapping population, and only 4 primer pairs were demonstrated to produce fragments of expected size.
Of the 4 products, 3 (CWGATT01A, CSWGATT01C and CSWTAAA01) were mapped to the same linkage group by use of MAPMAKER/EXP, version 3.0b, and de was illustrated in the linkage group located between CSWGATT01C and CSWTAAA01 at 9.5 and 21.5 cM from the markers, respectively.
Publication
Authors
Ming Xin, Zhiwei Qin
Keywords
Cucumis sativus L, inheritance, SSR, blunted with blossom
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