Articles
THE DIFFERENTIAL EXPRESSION OF RESISTANT GENES RELATED TO CUCUMBER SCAB DISPLAYED BY CDNA-AFLP
Article number
871_89
Pages
649 – 656
Language
English
Abstract
In order to understand the molecular basis of the inheritance of scab-resistance and provide basic information for disease-resistant breeding in cucumber.
The seedlings of resistant inbred line HX613 and susceptible inbred line HX36 were inoculated by spraying the pathogenic suspension of Cladosporium cucumerium with sterilized water as control.
After 6, 12, 24, 48, 72, 96, and 146 h of inoculation, the covered tender leaves from the plants of different treatments were sampled.
RNA was extracted from the leaves and reverse-transcribed to cDNA. The differential expression of genes resistant-related to scab was analyzed by cDNA-AFLP. Two hundred sixteen TDFs (Transcriptic derived fragment) were screened out.
Among them, 27 TDFs was enhanced expression, and the expression of 12 TDFs was weakened, 154 TDFs was transient expression and 23 TDFs was a constitutive expression, accounting for 12.5, 5.6, 71.3 and 10.6% respectively.
We cloned and sequenced 8 TDFs which expressed strongly and repeatedly by same primers.
Homology analysis indicated that TDF E11M59104 was related to the gene participating in defense reaction and substance decomposition in cell; TDF E11M59489 was homologous to pathogenesis- related proteins; E25M63203 is 96% homologous with resistant protein.
Other 5 TDFs were involved in cell-cycle regulation, transmembrane movements and metabolism etc.
The above results showed that the resistant reaction of cucumber to scab dealt with multiple aspects of pathology, physiology, biochemistry and molecular biology in the interaction of the plant and pathogen.
The seedlings of resistant inbred line HX613 and susceptible inbred line HX36 were inoculated by spraying the pathogenic suspension of Cladosporium cucumerium with sterilized water as control.
After 6, 12, 24, 48, 72, 96, and 146 h of inoculation, the covered tender leaves from the plants of different treatments were sampled.
RNA was extracted from the leaves and reverse-transcribed to cDNA. The differential expression of genes resistant-related to scab was analyzed by cDNA-AFLP. Two hundred sixteen TDFs (Transcriptic derived fragment) were screened out.
Among them, 27 TDFs was enhanced expression, and the expression of 12 TDFs was weakened, 154 TDFs was transient expression and 23 TDFs was a constitutive expression, accounting for 12.5, 5.6, 71.3 and 10.6% respectively.
We cloned and sequenced 8 TDFs which expressed strongly and repeatedly by same primers.
Homology analysis indicated that TDF E11M59104 was related to the gene participating in defense reaction and substance decomposition in cell; TDF E11M59489 was homologous to pathogenesis- related proteins; E25M63203 is 96% homologous with resistant protein.
Other 5 TDFs were involved in cell-cycle regulation, transmembrane movements and metabolism etc.
The above results showed that the resistant reaction of cucumber to scab dealt with multiple aspects of pathology, physiology, biochemistry and molecular biology in the interaction of the plant and pathogen.
Publication
Authors
Sun Xun, Li Xixiang, Qiu Yang, Shen Di, Song Jiangping, Ren Ruixing
Keywords
cucumber, differential expression, resistant gene, scab, cDNA-AFLP
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