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Articles

UTILIZATION OF NOVEL GENOTYPE OF BOTTLE GOURD FOR IDENTIFICATION OF HYBRID SEED WITH RAPD MARKER

Article number

871_68

Pages

491 – 498

Language

English

Abstract

RAPD markers of all five parental genotypes were discretely amplified and enough polymorphism was evident among them.
A total of 22 DNA bands were amplified ranging from minimum one unique band in PBOG-54 (segmented leaf, deeply lobed leaf) to a maximum of 7 DNA bands in PBOG 61. The genotype PBOG 22 displayed 6 characteristics DNA band followed by Pusa Naveen having 5 bands and PBOG 13 with 3 bands.
The entire profile ranged from 600 to 1900 bp.
The two F1 hybrids having common parent PBOG 54 were also subjected to RAPD analysis with similar protocol.
The genotypes involved in a cross produced amplification true to their phenotype which was similar to parental pattern.
RAPD markers clearly identified the genotypes and amplified their individual band pattern over the generation especially the critical single and dominant band in case of segmented leaf genotype PBOG-54. This indicates the possibility of RAPD marker to be used for detection of hybrid seed from selfed seed in a seed mixture for bottle gourd.

Publication

IV International Symposium on Cucurbits

Authors

D.K. Singh, S. Padiyar, H. Choudhary, A. Tiwari, H.H. Ram

Keywords

Lagenaria siceraria, segmented leaf, DNA marker, morphological marker, seed purity

Full text

https://doi.org/10.17660/ActaHortic.2010.871.68

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