Home > IV International Symposium on Cucurbits > CHARACTERIZATION OF NBS-LRR RESISTANCE GENE ANALOGS FROM A HIGH RESISTANCE TO DOWNY MILDEW INTROGRESSION LINE FROM CUCUMIS HYSTRIX ×C. SATIVUS

Articles

CHARACTERIZATION OF NBS-LRR RESISTANCE GENE ANALOGS FROM A HIGH RESISTANCE TO DOWNY MILDEW INTROGRESSION LINE FROM CUCUMIS HYSTRIX ×C. SATIVUS

Article number

871_79

Pages

573 – 578

Language

English

Abstract

Nucleotide-Binding Site and Leucine-rich Repeat Region (NBS-LRR) are the largest class of disease resistance genes cloned from plants up to date.
In this paper, NBS-type resistance genes were isolated and cloned from a high resistance to downy mildew introgression line from C. hystrix ×C. sativus (IL5211S) using a PCR method with degenerate primers designed from conserved regions of the NBS domain.
A total of twenty eight sequences were identified in IL5211S and named as cucumber resistance gene analogues (CSRGAs). Polygenetic analysis classified these sequences into four subclasses (CSRGA I-IV). Isolation of these CSRGAs may prove to be useful for developing RGA markers and map-based cloning of R-genes in IL5211S.

Publication

IV International Symposium on Cucurbits

Authors

Hongjian Wan, Jinfeng Chen

Keywords

NBS-LRR, introgression line, Cucumis hystrix, Cucumis sativus

Full text

https://doi.org/10.17660/ActaHortic.2010.871.79

Online Articles (92)

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79. CHARACTERIZATION OF NBS-LRR RESISTANCE GENE ANALOGS FROM A HIGH RESISTANCE TO DOWNY MILDEW INTROGRESSION LINE FROM CUCUMIS HYSTRIX ×C. SATIVUS

Hongjian Wan | Jinfeng Chen

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81. THE EFFECTS OF PROLINE AND COCONUT WATER ON CALLUS INDUCTION OF CUCUMBER (CUCUMIS SATIVUS L.)

P. Tantasawat | W. Chaowiset | A. Sorntip | C. Kativat | A. Wannajindaporn

82. LATEST ADVANCES IN WATERMELON GENOMICS

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Hui Song | Jinfeng Chen | J. Staub | P. Simon

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GH. Peyvast | J.A. Olfati | M. Piri | M.B. Mahdieh

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A.A. Glala | E.H. Abd El-Samad | S.M. Adam | Y.I. Helmy

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Aimin Wei | Shengli Du | Yike Han | Nan Liu | Guihua Zhang

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HongBing Fu | ShuPing Qu | ChongShi Cui | ZhuGang Li | Xi Zhao

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