STUDIES ON MITOTIC CHROMOSOME PREPARATION AND KARYOTYPE ANALYSIS OF TWO DIFFERENT GROUPS OF MELON IN CHINA

The effect of different sampling time and treating chemicals on chromosome preparation of root tip cells of melon was studied.
After sampling at 8:00 in morning, which is the best one of sampling time tested, there are up to 14% of cells observed in metaphase.
The efficiency of four pre-treating chemicals were compared, the best one was a mixture of two chemicals, cycloheximide (CHX, 40 mg•L^-1) and p-dichlorobenzene (Pdb, saturated), after pretreatment with the mixture clearly morphological and well distributed chromosomes were observed in metaphase which indicated that would be good for karyotype analysis in melon.
Metaphase chromosomes of two different groups of melon in China, thick and thin rind melon, were analyzed by the modified techniques of chromosome preparation.
The results are as followed: the karyotype formula of two groups are the same, 2n=2x=24 =14 m+10 st (2 SAT), and the karyotype are 2A type, there are a pare of satellite chromosomes in both.
Otherwise, the total and average length of chromosome, the satellite chromosomes of the two groups were different.

IV International Symposium on Cucurbits

Yongbing Zhang, Mingzhu Wu, Hongping Yi, Yan Zhang, Wenqiang Zhai, Jiongxin Feng

melon, mitosis, chromosome, karyotype

https://doi.org/10.17660/ActaHortic.2010.871.91