Articles
IMPROVEMENT OF THE PROPAGATION OF GLADIOLUS HYBRIDS SELECTED FOR EXTRA-SEASON MEDITERRANEAN PRODUCTION
Article number
886_30
Pages
219 – 224
Language
English
Abstract
Gladiolus, is a very popular species blooming late spring and summer for gardening and cut flower production.
New hybrids have been recently obtained by a Spanish-Italian breeding group with the aim to increase the offer of extra-season flowers with the production in winter and early spring in countries bordering the Mediterranean sea.
In order to speed up the offer of the new hybrids by a fast propagation system and to produce a stock of virus-free mother plant, a combined protocol was established using in vitro propagation by simple bioreactors and in vivo corm enlargement and multiplication.
Aseptic explants were achieved after meristem tip excision and the shoots were firstly multiplied in MS medium containing 2ip (2.98 µM). Cormels were then transferred to RITA® (Vitropic, CIRAD, France) vessels for Temporary Immersion culture with supply of an IBA (4.90 µM) containing medium.
Three minutes of flooding every three hours were set up and the vessels were cultured at 23°C at a 16-h photoperiod at 35 µE m-2 s-1. Two months later the cormels produced in TIS were very easily separated and then dried in a laminar flow cabinet for 24 hours and were stored at 5°C for 6 months.
After sowing in field an emergence of 100% was detected and after the first blooming a big number of cormels developed from the new corm depending on the genotype and on the depth of plantation.
New hybrids have been recently obtained by a Spanish-Italian breeding group with the aim to increase the offer of extra-season flowers with the production in winter and early spring in countries bordering the Mediterranean sea.
In order to speed up the offer of the new hybrids by a fast propagation system and to produce a stock of virus-free mother plant, a combined protocol was established using in vitro propagation by simple bioreactors and in vivo corm enlargement and multiplication.
Aseptic explants were achieved after meristem tip excision and the shoots were firstly multiplied in MS medium containing 2ip (2.98 µM). Cormels were then transferred to RITA® (Vitropic, CIRAD, France) vessels for Temporary Immersion culture with supply of an IBA (4.90 µM) containing medium.
Three minutes of flooding every three hours were set up and the vessels were cultured at 23°C at a 16-h photoperiod at 35 µE m-2 s-1. Two months later the cormels produced in TIS were very easily separated and then dried in a laminar flow cabinet for 24 hours and were stored at 5°C for 6 months.
After sowing in field an emergence of 100% was detected and after the first blooming a big number of cormels developed from the new corm depending on the genotype and on the depth of plantation.
Authors
B. Ruffoni, M. Pamato, M. Brea
Keywords
in vitro culture, cormels, Temporary Immersion System
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