Articles
THE USE OF ELISA IN THE MICROPROPAGATION OF VIRUS-FREE NARCISSUS
Article number
886_35
Pages
253 – 258
Language
English
Abstract
The aim of the research was to obtain virus-free in vitro cultures of narcissus (Narcissus L.) to establish elite plantations of Polish cultivars.
For this reason, selection of healthy donor plants, indexing during in vitro culture and virus eradication were carried out.
In an earlier study, potyvirus-free narcissus plants were obtained from infected bulbs using in vitro propagation via adventitious shoot regeneration (Sochacki and Orlikowska, 2005). Plants without symptoms of viral diseases of old and new, large-cupped and trumpet, Polish cultivars, and breeding clones of daffodil were used for the initiation of in vitro shoot cultures.
Enzyme Linked ImmunoSorbent Assays (ELISA) were used to detect the following viruses: Arabis mosaic (ArMV), Cucumber mosaic (CMV), Narcissus latent (NLV), Narcissus mosaic (NMV) and the group of potyviruses.
The tested plants were mostly infected with the NLV. The Narcissus mosaic virus and potyviruses were also detected.
Indivisible virus-negative clones were also selected by indexing during in vitro culture from narcissus plants infected with the NMV and NLV. The results showed that the process of micropropagation via adventitious shoot regeneration made it possible to eliminate the viruses from the infected narcissus plants but it all depended on the particular virus and its concentration in the plants.
In the case of one cultivar, almost totally infected with the NMV, an experiment to eradicate the virus using ribavirin (virazole) was conducted.
The antiviral compound used at concentrations of 12.5 or 25 mg/L resulted in the indexing of four plantlets as virus-negative.
For this reason, selection of healthy donor plants, indexing during in vitro culture and virus eradication were carried out.
In an earlier study, potyvirus-free narcissus plants were obtained from infected bulbs using in vitro propagation via adventitious shoot regeneration (Sochacki and Orlikowska, 2005). Plants without symptoms of viral diseases of old and new, large-cupped and trumpet, Polish cultivars, and breeding clones of daffodil were used for the initiation of in vitro shoot cultures.
Enzyme Linked ImmunoSorbent Assays (ELISA) were used to detect the following viruses: Arabis mosaic (ArMV), Cucumber mosaic (CMV), Narcissus latent (NLV), Narcissus mosaic (NMV) and the group of potyviruses.
The tested plants were mostly infected with the NLV. The Narcissus mosaic virus and potyviruses were also detected.
Indivisible virus-negative clones were also selected by indexing during in vitro culture from narcissus plants infected with the NMV and NLV. The results showed that the process of micropropagation via adventitious shoot regeneration made it possible to eliminate the viruses from the infected narcissus plants but it all depended on the particular virus and its concentration in the plants.
In the case of one cultivar, almost totally infected with the NMV, an experiment to eradicate the virus using ribavirin (virazole) was conducted.
The antiviral compound used at concentrations of 12.5 or 25 mg/L resulted in the indexing of four plantlets as virus-negative.
Authors
D. Sochacki
Keywords
bulbs, daffodil, in vitro, potyvirus, ribavirine, viral diseases, virus eradication
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