Articles
CURCUMA ALISMATIFOLIA GAGNEP. MICROPROPAGATION IN TWIN-FLASKS TEMPORARY IMMERSION BIOREACTOR
Article number
886_37
Pages
267 – 271
Language
English
Abstract
A micropropagation system of Curcuma alismatifolia Gagnep. cultured in twin-flasks temporary immersion bioreactors (TIB) was investigated.
This particular study included initiation, multiplication and rooting stages.
Curcuma inflorescences, used as initial explants, were cultured on a modified MS (1962) solid medium supplemented with 10 mg/L BA for 8 weeks.
After that reverted explants were then transferred to TIB with the same medium but lower BA concentration (1 mg/L) to improve the initiation system, compared with solid medium.
During the multiplication stage, four different durations and feeding frequencies of TIB namely 2 1-minute feeding/day; 6 1-minute/day; 2 15-minute/day; and 6 15-minute/day were investigated using a modified MS (1962) medium supplementary with 0.5 mg/L thidiazuron and 4 mg/L Imazalil.
These treatments were compared with those cultured on solid and liquid media for a 6-week period.
Results showed that retarded shoots cultured in TIB with 6-time feeding/day for either 1 or 15 minutes gave a high number ranging from 1,263 to 1,485 shoots per container.
On the contrary, shoots cultured on the solid medium produced only 285 shoots per container.
Retarded shoots from TIB were then transferred to TIB containing modified MS medium without hormones for elongation and rooting.
It was noticed that retarded shoots were elongated and rooted with different sizes within 4 weeks.
Finally these shoots were selected for uniformity.
This particular study included initiation, multiplication and rooting stages.
Curcuma inflorescences, used as initial explants, were cultured on a modified MS (1962) solid medium supplemented with 10 mg/L BA for 8 weeks.
After that reverted explants were then transferred to TIB with the same medium but lower BA concentration (1 mg/L) to improve the initiation system, compared with solid medium.
During the multiplication stage, four different durations and feeding frequencies of TIB namely 2 1-minute feeding/day; 6 1-minute/day; 2 15-minute/day; and 6 15-minute/day were investigated using a modified MS (1962) medium supplementary with 0.5 mg/L thidiazuron and 4 mg/L Imazalil.
These treatments were compared with those cultured on solid and liquid media for a 6-week period.
Results showed that retarded shoots cultured in TIB with 6-time feeding/day for either 1 or 15 minutes gave a high number ranging from 1,263 to 1,485 shoots per container.
On the contrary, shoots cultured on the solid medium produced only 285 shoots per container.
Retarded shoots from TIB were then transferred to TIB containing modified MS medium without hormones for elongation and rooting.
It was noticed that retarded shoots were elongated and rooted with different sizes within 4 weeks.
Finally these shoots were selected for uniformity.
Authors
N. Topoonyanont, S. Jaikanta, P. Boonmanee
Keywords
curcuma, automation, temporary immersion bioreactor, micropropagation
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