Articles
HISTOGENIC IDENTIFICATION BY CYTOLOGICAL ANALYSIS OF COLCHICINE-INDUCED POLYPLOIDS OF HEMEROCALLIS
Article number
886_34
Pages
247 – 252
Language
English
Abstract
Tetraploids have taken a leading position among the daylilies cultivars due to the desirable traits such as vigorous growth and large flower.
The objective of this study was to determine the ploidy level in colchicine-induced plants and to identify ploidy chimeras based on 1) stomata length, 2) leaf width and 3) chromosome number in root tip cells.
These three parameters show the ploidy level in the layer L1, L2 and L3, respectively.
Stomata lengths and chromosome counts were estimated in diploids (2n=2x=22; Hemerocallis × hybrid Blink of an Eye, H. multiflora Berliner Multi), tetraploid (Beyond New Millenium), periclinal chimeras 2-4-4 (Tetra Siloam Double Classic, Tetra Janice Brown) and colchicine-induced plants.
The stomata length depends not only on the ploidy level but also on the leaf age and the stomata position on the leaf.
The stomata from the fourth leaf, more distant from the tip, 15-20 cm, were generally longer than those from the third leaf, 10 cm distant from the tip.
Therefore, the leaf sampling for the comparative determination of the ploidy level should be well defined.
Independently of the genotype, the stomata length was strongly associated with the ploidy level.
Diploids as well as chimeras 2-4-4 had shorter stomata than tetraploids by 30%. Each colchicine-treated diploids produced four or five regenerants, and about 50% of them differed from mother plants.
They had wider leaves and longer stomata.
Such stomata were observed all over the leaf blade area or they occurred only in some leaf sectors.
The newly-obtained plants were recognized as sectorial chimeras.
The objective of this study was to determine the ploidy level in colchicine-induced plants and to identify ploidy chimeras based on 1) stomata length, 2) leaf width and 3) chromosome number in root tip cells.
These three parameters show the ploidy level in the layer L1, L2 and L3, respectively.
Stomata lengths and chromosome counts were estimated in diploids (2n=2x=22; Hemerocallis × hybrid Blink of an Eye, H. multiflora Berliner Multi), tetraploid (Beyond New Millenium), periclinal chimeras 2-4-4 (Tetra Siloam Double Classic, Tetra Janice Brown) and colchicine-induced plants.
The stomata length depends not only on the ploidy level but also on the leaf age and the stomata position on the leaf.
The stomata from the fourth leaf, more distant from the tip, 15-20 cm, were generally longer than those from the third leaf, 10 cm distant from the tip.
Therefore, the leaf sampling for the comparative determination of the ploidy level should be well defined.
Independently of the genotype, the stomata length was strongly associated with the ploidy level.
Diploids as well as chimeras 2-4-4 had shorter stomata than tetraploids by 30%. Each colchicine-treated diploids produced four or five regenerants, and about 50% of them differed from mother plants.
They had wider leaves and longer stomata.
Such stomata were observed all over the leaf blade area or they occurred only in some leaf sectors.
The newly-obtained plants were recognized as sectorial chimeras.
Authors
M. Podwyszyńska, E. Gabryszewska, D. Sochacki, A. Jasiński
Keywords
Hemerocallis, polyploid, tetraploid, chimera, stomatal length, colchicine
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