Articles
ISOLATION OF TWO UBIQUITIN PROMOTERS FROM GLADIOLUS
Article number
886_46
Pages
323 – 328
Language
English
Abstract
Two ubiquitin promoters from Gladiolus, GUBQ2 and GUBQ4, were isolated by screening multiple phage genomic DNA libraries with RUBQ2, an ubiquitin gene from rice.
GUBQ2 was identified as a polyubiquitin promoter because of its ubiquitin gene monomers.
GUBQ2 contained the evolutionarily conserved 5 and 3 splice sites for its 682 bp intron in the conserved position immediately upstream from the ATG initiation codon.
This intron had a 68% AU content similar to the minimum 60% AU content reported for dicots.
The uidA gene that codes for GUS gene expression was subcloned under either the GUBQ2 or GUBQ4 promoters and used to transform callus by biolistics.
Putatively transformed Gladiolus plants were selected by visual, histochemical staining of callus, young leaves, and young roots.
Roots of some plant lines expressed GUS either throughout the length of the root or specifically in its meristem.
GUBQ2 was identified as a polyubiquitin promoter because of its ubiquitin gene monomers.
GUBQ2 contained the evolutionarily conserved 5 and 3 splice sites for its 682 bp intron in the conserved position immediately upstream from the ATG initiation codon.
This intron had a 68% AU content similar to the minimum 60% AU content reported for dicots.
The uidA gene that codes for GUS gene expression was subcloned under either the GUBQ2 or GUBQ4 promoters and used to transform callus by biolistics.
Putatively transformed Gladiolus plants were selected by visual, histochemical staining of callus, young leaves, and young roots.
Roots of some plant lines expressed GUS either throughout the length of the root or specifically in its meristem.
Authors
K. Kamo, Y.H. Joung, K. Green
Keywords
introns, monocot flowers, genetic engineering
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