Articles
MA-ACS1: A KEY OPERATOR IN ETHYLENE BIOSYNTHESIS IN BANANA – ITS ROLE AND REGULATION DURING FRUIT RIPENING
Article number
897_20
Pages
187 – 194
Language
English
Abstract
MA-ACS1 is the major ripening gene in banana and plays a crucial role in the regulation of ethylene production during ripening.
Recently, we identified the cDNA for MA-ACS1 from banana and studied the transcript and protein accumulation patterns of this gene.
The expression of this gene correlated well with 1-aminocyclopropane 1-carboxylic acid (ACC) content in banana pulp during ripening.
In-silico analysis provided important insight in the structural and phylogenetic characteristics of MA-ACS1. We analysed the differential transcript accumulation pattern and protein levels of MA-ACS1 in response to ethylene, auxin, wounding and low temperature in preclimacteric banana fruit.
Our study provides information about the regulation of expression of the MA-ACS1 in response to various factors during ripening in banana fruit, which may have physiological relevance concerning ethylene biosynthesis during post-harvest conditions.
Furthermore, we have detected a GCC-box putative ERE (ethylene-responsive element) and an ARE (auxin-responsive element) specific DNA-binding activity in the banana pulp and studied the ethylene- and auxin-responsive characteristics of the GCC-box and ARE (TGTCTC)-containing synthetic promoter fragments.
Understanding the expression pattern of ripening genes and the molecular mechanisms that regulate gene expression during ripening is crucial in regulating the expression of ripening genes to control ripening under post-harvest conditions to reduce massive spoilage of fruits during shipping and storage.
Recently, we identified the cDNA for MA-ACS1 from banana and studied the transcript and protein accumulation patterns of this gene.
The expression of this gene correlated well with 1-aminocyclopropane 1-carboxylic acid (ACC) content in banana pulp during ripening.
In-silico analysis provided important insight in the structural and phylogenetic characteristics of MA-ACS1. We analysed the differential transcript accumulation pattern and protein levels of MA-ACS1 in response to ethylene, auxin, wounding and low temperature in preclimacteric banana fruit.
Our study provides information about the regulation of expression of the MA-ACS1 in response to various factors during ripening in banana fruit, which may have physiological relevance concerning ethylene biosynthesis during post-harvest conditions.
Furthermore, we have detected a GCC-box putative ERE (ethylene-responsive element) and an ARE (auxin-responsive element) specific DNA-binding activity in the banana pulp and studied the ethylene- and auxin-responsive characteristics of the GCC-box and ARE (TGTCTC)-containing synthetic promoter fragments.
Understanding the expression pattern of ripening genes and the molecular mechanisms that regulate gene expression during ripening is crucial in regulating the expression of ripening genes to control ripening under post-harvest conditions to reduce massive spoilage of fruits during shipping and storage.
Publication
Authors
S. Roy Choudhury, S. Roy, S.K. Singh, D.N. Sengupta
Keywords
ACC synthase, amino transferase, auxin, climacteric, Musa
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