Articles
ANALYSIS OF GENETIC DIVERSITY IN BANANA CULTIVARS (MUSA SPP.) USING SEQUENCE-RELATED AMPLIFIED POLYMORPHISM MARKERS
Article number
897_30
Pages
263 – 265
Language
English
Abstract
Most cultivated banana (Musa spp.) have evolved from interspecific hybrids of two wild diploid species, Musa acuminate Colla (AAw genome) and Musa balbisiana Colla (BBw genome). Molecular marker techniques can facilitate characterisation and differentiation of banana populations.
In this paper, a novel molecular marker technique known as sequence-related amplified polymorphism (SRAP) was studied to determine diversity and relationships among 29 banana genotypes.
The results showed that 25 out of 64 primer combinations were polymorphic and 324 polymorphic bands were obtained.
Based on the appearance of the markers, the genetic relationships were analysed by the unweighted pair-group method of arithmetic average (UPGMA) cluster analysis.
The results showed that most genetic relationships among cultivars were correlated to their region of origin.
All 29 cultivars could be clustered into two major clusters closely corresponding with the genome composition of the genotypes (AA genome and hybrids between AA and BB genome). Some cultivars examined in this study did not cluster according to their hypothetical genetic homologies, which would suggest missampling or misidentification.
All results indicated that the amplification of SRAP markers is useful for estimating genetic similarity among banana genotypes.
In this paper, a novel molecular marker technique known as sequence-related amplified polymorphism (SRAP) was studied to determine diversity and relationships among 29 banana genotypes.
The results showed that 25 out of 64 primer combinations were polymorphic and 324 polymorphic bands were obtained.
Based on the appearance of the markers, the genetic relationships were analysed by the unweighted pair-group method of arithmetic average (UPGMA) cluster analysis.
The results showed that most genetic relationships among cultivars were correlated to their region of origin.
All 29 cultivars could be clustered into two major clusters closely corresponding with the genome composition of the genotypes (AA genome and hybrids between AA and BB genome). Some cultivars examined in this study did not cluster according to their hypothetical genetic homologies, which would suggest missampling or misidentification.
All results indicated that the amplification of SRAP markers is useful for estimating genetic similarity among banana genotypes.
Publication
Authors
J.Y. Wei, D.B. Liu, S.X. Wei, Z.S. Xie, G.Y. Xu, Y.Y. Chen
Keywords
SRAP markers, genetic relationship
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