Articles
DIFFERENT APPROACHES TO DISCOVER THE REPETITIVE PART OF THE BANANA NUCLEAR GENOME
Article number
897_5
Pages
63 – 68
Language
English
Abstract
Banana and plantain (Musa spp.), hereafter called banana, are one of the most important crops in the world.
They provide the staple diet for millions people in tropical and subtropical countries.
Unfortunately, banana production is threatened by many diseases and pests.
Edible banana cultivars are seed-sterile, vegetatively propagated crops, which complicates traditional breeding.
Despite the relatively small genome (1C = 550-650 Mbp), the structure and organisation of the banana nuclear genome and genetic diversity of Musa is still largely unknown.
In this review, we focus on the repetitive part of the banana genome.
Although repeats have been called junk or selfish DNA, at least some of the repeats have well defined functions.
Repeats are ubiquitous, can evolve more rapidly than genic sequences in the genome and are frequently used for developing molecular markers.
Characterisation of species-specific repeats and the creation of a banana repeat database would be useful for repeat identification and repeat masking during the sequencing project.
Moreover, the knowledge of banana repetitive elements, especially satellite DNA and genome-specific repeats, could be useful for identification of individual chromosomes or different genomes in banana interspecific hybrids.
They provide the staple diet for millions people in tropical and subtropical countries.
Unfortunately, banana production is threatened by many diseases and pests.
Edible banana cultivars are seed-sterile, vegetatively propagated crops, which complicates traditional breeding.
Despite the relatively small genome (1C = 550-650 Mbp), the structure and organisation of the banana nuclear genome and genetic diversity of Musa is still largely unknown.
In this review, we focus on the repetitive part of the banana genome.
Although repeats have been called junk or selfish DNA, at least some of the repeats have well defined functions.
Repeats are ubiquitous, can evolve more rapidly than genic sequences in the genome and are frequently used for developing molecular markers.
Characterisation of species-specific repeats and the creation of a banana repeat database would be useful for repeat identification and repeat masking during the sequencing project.
Moreover, the knowledge of banana repetitive elements, especially satellite DNA and genome-specific repeats, could be useful for identification of individual chromosomes or different genomes in banana interspecific hybrids.
Publication
Authors
E. Hřibová, J. Doležel
Keywords
Cot filtration, DNA sequencing, massively parallel sequencing technologies, Musa, nuclear genome, repetitive elements
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