Articles
GENE EXPRESSION ANALYSIS IN ROOTS OF MUSA ACUMINATE SSP. BURMANNICOIDES ‘CALCUTTA-4’, A BANANA GENOTYPE TOLERANT TO FUSARIUM WILT
Article number
897_49
Pages
363 – 370
Language
English
Abstract
Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense (Foc), is one of the most devastating diseases of banana.
In India, most banana cultivars are highly susceptible to this pathogen.
In this study, wild Musa acuminate ssp. burmannicoides Calcutta-4 (tolerant) and cultivar Kadali (AA, susceptible) were infected with Foc in pot experiments. Calcutta-4 showed significantly less disease severity symptoms than Kadali, which showed symptoms like early yellowing of leaves, browning of roots and vascular discoloration.
Suppression subtractive hybridisation was carried out to identify genes induced in roots of Calcutta-4 after infection with Foc.
One hundred and nine (109) clones were sequenced and 68 non-redundant sequences were obtained.
They were characterised based on homology search in the National Center for Biotechnology Information (NCBI) database.
Some cDNAs which showed homology to defence-related genes, like PGIP2, peroxidase and catalase that help in cell-wall strengthening; S-adenosyl methionine synthetase and other methyl transferases that play a role in phenylpropanoid biosynthesis and also genes that are involved in signal transduction, were identified.
In India, most banana cultivars are highly susceptible to this pathogen.
In this study, wild Musa acuminate ssp. burmannicoides Calcutta-4 (tolerant) and cultivar Kadali (AA, susceptible) were infected with Foc in pot experiments. Calcutta-4 showed significantly less disease severity symptoms than Kadali, which showed symptoms like early yellowing of leaves, browning of roots and vascular discoloration.
Suppression subtractive hybridisation was carried out to identify genes induced in roots of Calcutta-4 after infection with Foc.
One hundred and nine (109) clones were sequenced and 68 non-redundant sequences were obtained.
They were characterised based on homology search in the National Center for Biotechnology Information (NCBI) database.
Some cDNAs which showed homology to defence-related genes, like PGIP2, peroxidase and catalase that help in cell-wall strengthening; S-adenosyl methionine synthetase and other methyl transferases that play a role in phenylpropanoid biosynthesis and also genes that are involved in signal transduction, were identified.
Publication
Authors
K.V. Ravishankar, A. Rekha, V. Swarupa, G. Savitha
Keywords
catalase, cDNA, peroxidase
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