Articles
GENERATION OF SEGREGATING F1 POPULATIONS FOR MAPPING THE MUSA GENOME
Article number
897_33
Pages
277 – 278
Language
English
Abstract
Banana cultivars have been classified into genome groups based on their morphological characters.
Modern molecular tools are available to support characterisation, identification of hybridisation and analysis of evolutionary trends.
Two wild species, Musa acuminate ssp. burmannicoides Cacutta-4 (female parent) and Musa balbisiana Bee Hee Kela (male parent) collected from Northeastern Hilly region of India, were crossed to produce F1 populations of the AB type with characters from the heterozygous parents.
Seed set was very high (3552 seeds) and 317 seedlings germinated.
About 150 seedlings were maintained for further evaluation and molecular mapping.
Plant height was dwarf to medium, similar to Calcutta-4. The pseudostem was green with few black-brown blotches, expressing both characters, but less waxy, which is characteristic of the A genome.
For the 52 hybrids that flowered, most bunches were horizontal, showing the AB character.
However, there was good segregation for male flower colour.
The colour varied from cream to pink-purple with gradations of pink (31% of the observed progeny). It was observed that 40% of the F1 population studied did not produce any pollen and was totally sterile, unlike the parent accessions.
These F1 populations could serve as useful material for studying segregation of different morphological characters and molecular mapping.
Modern molecular tools are available to support characterisation, identification of hybridisation and analysis of evolutionary trends.
Two wild species, Musa acuminate ssp. burmannicoides Cacutta-4 (female parent) and Musa balbisiana Bee Hee Kela (male parent) collected from Northeastern Hilly region of India, were crossed to produce F1 populations of the AB type with characters from the heterozygous parents.
Seed set was very high (3552 seeds) and 317 seedlings germinated.
About 150 seedlings were maintained for further evaluation and molecular mapping.
Plant height was dwarf to medium, similar to Calcutta-4. The pseudostem was green with few black-brown blotches, expressing both characters, but less waxy, which is characteristic of the A genome.
For the 52 hybrids that flowered, most bunches were horizontal, showing the AB character.
However, there was good segregation for male flower colour.
The colour varied from cream to pink-purple with gradations of pink (31% of the observed progeny). It was observed that 40% of the F1 population studied did not produce any pollen and was totally sterile, unlike the parent accessions.
These F1 populations could serve as useful material for studying segregation of different morphological characters and molecular mapping.
Publication
Authors
A. Rekha, K.V. Ravishankar, D.S. Ambika
Keywords
Online Articles (73)