Home > V International Symposium on Banana: ISHS-ProMusa Symposium on Global Perspectives on Asian Challenges > EARLY EVENTS IN THE FUSARIUM OXYSPORUM F. SP. CUBENSE ¿ MUSA INTERACTION USING GREEN FLUORESCENT PROTEIN-TAGGED RACE 4

Articles

EARLY EVENTS IN THE FUSARIUM OXYSPORUM F. SP. CUBENSE ¿ MUSA INTERACTION USING GREEN FLUORESCENT PROTEIN-TAGGED RACE 4

Article number

897_42

Pages

305 – 311

Language

English

Abstract

Fusarium oxysporum f. sp. cubense (Foc) is the causal pathogen of Fusarium wilt of banana.
To understand some of the early events in the Foc-Musa interaction, we created a green fluorescent protein (GFP)-tagged transformant and studied its pathogenesis using fluorescence microscopy and confocal laser scanning microscopy.
The study showed that: (i) Foc race 4 was capable of invading the epidermal cells of banana roots directly; (ii) Invasion sites included epidermal cells of root caps and in the elongation zone.
Our results showed that roots of Foc race 4-susceptible banana plants were well colonised with the pathogen and demonstrated that GFP-tagged Foc race 4 isolates present an effective tool to study banana root-fungus interactions.

Publication

V International Symposium on Banana: ISHS-ProMusa Symposium on Global Perspectives on Asian Challenges

Authors

C.Y. Li, G.J. Yi, B.Z. Huang, Y.R. Wei

Keywords

banana, Fusarium wilt, green fluorescent protein

Full text

https://doi.org/10.17660/ActaHortic.2011.897.42

Online Articles (73)

1. STATUS, CHALLENGES AND TRENDS OF CHINESE BANANA INDUSTRY

C.Y. Li | Y.R. Wei | Y.L. Wu | B.Z. Huang | G.J. Yi

2. NON-CONVENTIONAL BREEDING OF BANANA (MUSA SPP.)

3. FROM FUNDAMENTAL RESEARCH DISCOVERIES TO APPLICATIONS FOR BANANA IMPROVEMENT

4. GENOMICS, BANANA BREEDING AND SUPERDOMESTICATION

J.S. Heslop-Harrison

5. DIFFERENT APPROACHES TO DISCOVER THE REPETITIVE PART OF THE BANANA NUCLEAR GENOME

E. Hřibová | J. Doležel

6. IDENTIFICATION AND VALIDATION OF EST-DERIVED MOLECULAR MARKERS, TRAP AND VNTRS, FOR BANANA RESEARCH

7. WILD MUSACEAE SPECIES IN CHINA

M. Häkkinen

8. MUSA COLLECTION AND CHARACTERISATION IN CENTRAL AND EASTERN DR CONGO: A CHRONOLOGICAL OVERVIEW

9. MORPHOLOGICAL CHARACTERISATION OF EAST AFRICAN AAB AND AA DESSERT BANANAS (MUSA SPP.)

M. Onyango | D. Karamura | S. Keeley | R. Manshardt | D. Haymer

10. THE COMPLEMENTARITY OF FARMERS’ AND BOTANICAL DESCRIPTORS OF THE EAST AFRICAN HIGHLAND BANANA CULTIVARS (MUSA, AAA)

D. Karamura | A. Kiggundu | E. Karamura

11. STANDARDISED PROCEDURE FOR MUSA GERMPLASM CHARACTERISATION

12. DIVERSITY ANALYSIS IN INDIAN COOKING BANANAS (MUSA, ABB) THROUGH MORPHOTAXONOMIC AND MOLECULAR CHARACTERISATION

13. APPLICATION OF CYTOPLASMIC AND NUCLEAR DNA-BASED MARKER SYSTEMS FOR ELUCIDATION OF THE PHYLOGENETIC RELATIONSHIP OF MUSA ACUMINATE AND MUSA BALBISIANA AND THEIR HYBRIDS

R. Boonruangrod | D. Desai | M. Berenyi | S. Fluch | K. Burg

14. A COMPLEX APPROACH FOR UNRAVELLING MUSACEAE PHYLOGENY AT MOLECULAR LEVEL

P. Němcová | E. Hřibová | M. Valárik | J. Doležel

15. MOLECULAR ANALYSIS REVEALS MULTIPLE DOMESTICATIONS OF EDIBLE BANANAS

H. Volkaert

16. MICROSATELLITE MARKERS FOR CLASSIFYING AND ANALYSING GENETIC RELATIONSHIP BETWEEN BANANA CULTIVARS IN INDONESIA

A. Retnoningsih | R. Megia | A. Hartana

17. VALIDATION OF RAPID (COLOUR-BASED) PRESCREENING TECHNIQUES FOR ANALYSIS OF FRUIT PROVITAMIN A CONTENTS IN BANANA (MUSA SPP.)

L.C. Pereira | G.B. Ngoh Newilah | M.W. Davey | I. Van den Bergh

18. VITAMIN A BIOFORTIFICATION IN MUSA: STATUS, BOTTLENECKS AND PROSPECTS

M.W. Davey | I. Van den Bergh | N. Roux

19. CANDIDATE RESISTANCE GENE DISCOVERY: RESISTANCE GENE ANALOG CHARACTERISATION AND DIFFERENTIAL GENE EXPRESSION ANALYSIS IN MUSA-MYCOSPHAERELLA HOST-PATHOGEN INTERACTIONS

R.N.G. Miller | M.A.N. Passos | F.L. Emediato | C. de Camargo Teixeira | G.J. Pappas Júnior

20. MA-ACS1: A KEY OPERATOR IN ETHYLENE BIOSYNTHESIS IN BANANA – ITS ROLE AND REGULATION DURING FRUIT RIPENING

S. Roy Choudhury | S. Roy | S.K. Singh | D.N. Sengupta

21. COMBINATION OF SUPPRESSION-SUBTRACTIVE HYBRIDISATION WITH CDNA MICROARRAY, A NOVEL WAY TO IDENTIFY GENES FROM BANANA INVOLVED IN FRUIT RIPENING

Z.Q. Jin | B.Y. Xu | J.H. Liu | J.B. Zhang | C.H. Jia

22. DEVELOPING RESISTANT BANANA AND PLANTAIN CULTIVARS THROUGH CONVENTIONAL BREEDING TECHNIQUES

23. EXPLOITATION OF DIPLOIDS IN INDIAN BANANA BREEDING PROGRAMMES

S. Uma | M.M. Mustaffa | M.S. Saraswathi | P. Durai

24. THE EFFECT OF BANANA BREEDING ON THE DIVERSITY OF EAST AFRICAN HIGHLAND BANANA (MUSA, AAA)

M. Nyine | M. Pillay

25. USE OF MOLECULAR MARKERS IN BANANA AND PLANTAIN IMPROVEMENT

J. Lorenzen | S. Hearne | G. Mbanjo | M. Nyine | T. Close

26. THE USE OF MOLECULAR MARKERS IN THE CIRAD BANANA BREEDING PROGRAMME

J.P. Horry

27. DEVELOPMENT OF HIGHLY REGENERATIVE EMBRYOGENIC CELL SUSPENSIONS OF ‘NANJANGUD RASBALE’ (SYN. ‘RASTHALI’, MUSA, AAB, SILK SUBGROUP) AND TRANSFORMANTS WITH AMP GENE

28. COLLECTING AND CONSERVING WILD MUSA GERMPLASM IN GUANGXI, CHINA

J.Y. Yan | Q.X. Quan | X. Long | H.X. Peng

29. PRELIMINARY STUDY ON ISSR ANALYSIS AND CLASSIFICATION OF WILD MUSA GERMPLASM IN GUANGXI, CHINA

X.Q. Qin | H.X. Peng | X. Long | J.Y. Yao

30. ANALYSIS OF GENETIC DIVERSITY IN BANANA CULTIVARS (MUSA SPP.) USING SEQUENCE-RELATED AMPLIFIED POLYMORPHISM MARKERS

31. INTERSECTIONAL RELATIONSHIP BETWEEN EUMUSA AND RHODOCHLAMYS OF THE GENUS MUSA USING MORPHOTAXONOMY AND MICROSATELLITE MARKERS

P. Durai | S. Uma | M.S. Saraswathi | N. Jayabalan | M.M. Mustaffa

32. STUDIES ON GENETIC DIVERGENCE OF SEEDED BANANA IN WEST BENGAL

33. GENERATION OF SEGREGATING F₁ POPULATIONS FOR MAPPING THE MUSA GENOME

A. Rekha | K.V. Ravishankar | D.S. Ambika

34. GENE EXPRESSION ANALYSIS IN LEAVES OF ‘BEE HEE KELA’, A DROUGHT-TOLERANT MUSA BALBISIANA GENOTYPE FROM NORTHEAST INDIA

K.V. Ravishankar | A. Rekha | R.H. Laxman | G. Savitha | V. Swarupa

35. STUDIES ON TIME-COURSE EXPRESSION OF DEFENCE GENES IN BANANA AGAINST PRATYLENCHUS COFFEAE FOR THE CREATION OF A SUBTRACTIVE CDNA LIBRARY

36. SEED AS AN ALTERNATIVE SOURCE OF DNA FOR MOLECULAR RESEARCH OF INACCESSIBLE WILD MUSA SPECIES

S. Uma | M.S. Saraswathi | D. Anto

37. USING AN IMPROVED SITE-DIRECTED MUTAGENESIS FOR THE CREATION OF CONSTRUCTS TO ANALYSE GENE FUNCTION IN FUSARIUM OXYSPORUM F. SP. CUBENSE

38. TRANSFORMATION OF THE BANANA CULTIVAR ‘NANJANGUD RASBALE’ (SYN. ‘RASTHALI’, AAB, SILK SUBGROUP) WITH THE AMP GENE AND SCREENING FOR FUSARIUM RESISTANCE WITH A BIOASSAY

39. EVALUATION AND TISSUE-CULTURE CONSERVATION, MULTIPLICATION AND DISTRIBUTION OF RARE AND CAROTENOID-RICH FE’I BANANA CULTIVARS IN MICRONESIA

V.M. Verma

40. MICROPROPAGATION OF MALAYSIAN BANANA CULTIVARS (MUSA SPP.) USING A MODIFIED TWIN FLASK SYSTEM

L.K. Chan | V.H. Au

41. THE EFFECT OF ETHYL METHANE SULPHOMATE (EMS) AND SODIUM AZIDE (NAN₃) ON PLANT REGENERATION CAPACITY OF AN EMBRYOGENIC CELL SUSPENSION OF ‘YUEYOUKANG 1’ (MUSA, AAA), A BANANA CULTIVAR RESISTANT TO FUSARIUM WILT

C.X. Xu | J. Xiao | J.G. He | G.B. Hu | H.B. Chen

42. EARLY EVENTS IN THE FUSARIUM OXYSPORUM F. SP. CUBENSE ¿ MUSA INTERACTION USING GREEN FLUORESCENT PROTEIN-TAGGED RACE 4

C.Y. Li | G.J. Yi | B.Z. Huang | Y.R. Wei

43. INCIDENCE AND DISTRIBUTION OF FUSARIUM WILT DISEASE OF BANANA IN INDONESIA

44. STATUS OF FUSARIUM WILT IN INDIA

M.M. Mustaffa | R. Thangavelu

45. RAISING AWARENESS OF THE THREAT OF FUSARIUM WILT TROPICAL RACE 4 IN LATIN AMERICA AND THE CARIBBEAN

L.E. Pocasangre | R.C. Ploetz | A.B. Molina | L. Perez Vicente

46. A DETECTION METHOD FOR TROPICAL RACE 4 OF THE BANANA PATHOGEN FUSARIUM OXYSPORUM F. SP. CUBENSE

47. VEGETATIVE COMPATIBILITY GROUP ANALYSIS OF INDONESIAN FUSARIUM OXYSPORUM F. SP. CUBENSE ISOLATES

W.T. O'Neill | A.B. Pattison | J.W. Daniells | C. Hermanto | A.B. Molina

48. CROSS INFECTION AND GENETIC DIVERSITY OF FUSARIUM OXYSPORUM F. SP. CUBENSE, THE CAUSAL AGENT OF FUSARIUM WILT IN BANANA

R. Thangavelu | P. Suganya Devi | P.M. Chrismala | M.M. Mustaffa

49. GENE EXPRESSION ANALYSIS IN ROOTS OF MUSA ACUMINATE SSP. BURMANNICOIDES ‘CALCUTTA-4’, A BANANA GENOTYPE TOLERANT TO FUSARIUM WILT

K.V. Ravishankar | A. Rekha | V. Swarupa | G. Savitha

50. CHARACTERISATION OF EARLY EVENTS IN BANANA ROOTS INFECTED WITH GREEN FLUORESCENT PROTEIN-TAGGED FUSARIUM OXYSPORUM F. SP. CUBENSE

51. A GREENHOUSE BIOASSAY FOR THE FUSARIUM OXYSPORUM F. SP. CUBENSE X ‘GRAND NAINE’ (MUSA, AAA, CAVENDISH SUBGROUP) INTERACTION

M.A. Dita | C. Waalwijk | L.V. Paiva | M.T. Souza Jr. | G.H.J. Kema

52. DISCRIMINATION OF BANANA GENOTYPES FOR FUSARIUM WILT RESISTANCE IN THE GREENHOUSE

L.R. Ribeiro | E.P. Amorim | Z.J.M. Cordeiro | S. de Oliveira e Silva | M.A. Dita

53. REACTION OF DIPLOID (AA) AND TETRAPLOID (AAAB) BANANA HYBRIDS TO FUSARIUM WILT UNDER FIELD CONDITIONS

A.P. de Matos | Z.J.M. Cordeiro | S. de Oliveira e Silva | E.P. Amorim | D.M.V. Ferreira

54. SELECTION OF A NEW SOMACLONE CULTIVAR ‘TAI-CHIAO NO. 5’ (AAA, CAVENDISH) WITH RESISTANCE TO FUSARIUM WILT OF BANANA IN CHINESE TAIPEI

55. COMPARISON OF HOST REACTION TO FUSARIUM OXYSPORUM F. SP. CUBENSE TROPICAL RACE 4 AND AGRONOMIC PERFORMANCE OF SOMACLONAL VARIANT ‘GCTCV-119’ (AAA, CAVENDISH) AND ‘GRAND NAINE’ (AAA, CAVENDISH) IN COMMERCIAL FARMS IN THE PHILIPPINES

A.B. Molina | E.G. Fabregar | R.O. Soquita | V.G.O. Sinohin

56. COMBATING BANANA WILTS – WHAT DO RESISTANT CULTIVARS HAVE TO OFFER?

J.W. Daniells

57. FUSARIUM WILT INCIDENCE, GROWTH, YIELD AND POST-HARVEST QUALITY OF BANANA AS AFFECTED BY ORGANIC FARMING IN CHINESE TAIPEI

C.M. Chang | C.P. chao | S.N. Huang | S.C. Chiang

58. EFFICACY OF CLAY-BASED FORMULATED SERRATIA IN REDUCING INOCULUM OF FUSARIUM OXYSPORUM F. SP. CUBENSE TROPICAL RACE 4

A.S.Y. Ting | M.T. Fang | C.S. Tee

59. IN VITRO MUTATION INDUCTION FOR THE IMPROVEMENT OF MYCOSPHAERELLA FIJIENSIS RESISTANCE IN TWO BANANA CULTIVARS GROWN IN KENYA

M. Mwangi | S.W. Waudo | E.M. Kahangi

60. SCREENING MUSA GENOTYPES FOR BANANA BUNCHY TOP DISEASE RESISTANCE IN BURUNDI

C. Niyongere | E. Ateka | T. Losenge | G. Blomme | P. Lepoint

61. TRANSGENIC BANANA PLANTS RESISTANT TO BANANA BUNCHY TOP VIRUS INFECTION

62. ENHANCING BANANA CROP MANAGEMENT BY USE OF TISSUE-CULTURE DERIVED PLANTING MATERIAL IN KENYA

J. Njuguna | S. Nguthi | F. Wambugu | D. Gitau | M. Karuoya

63. VIRUS-INDEXING TECHNOLOGY FOR PRODUCTION OF QUALITY BANANA PLANTING MATERIAL: A BOON TO THE TISSUE-CULTURE INDUSTRY AND BANANA GROWERS IN INDIA

R. Selvarajan | V. Balasubramanian | M.M. Sheeba | R. Raj Mohan | M.M. Mustaffa

64. MONITORING FUSARIUM WILT IN HAINAN PROVINCE AND PRELIMINARY PATHOGEN CHARACTERISATION

X. Zhang | H. Zhang | Y. Xie | J. Pu | Y. Qi | Y. Lu

65. BANANA VARIETIES AND FUSARIUM OXYSPORUM F. SP. CUBENSE IN INDONESIA – OBSERVATIONS FROM FUSARIUM WILT DISEASE DATABASES

J.W. Daniells | W.T. O'Neill | C. Hermanto | R.C. Ploetz

66. EVALUATION OF SEEDED BANANA TYPES FOR BIOTIC STRESS

67. IMPORTANCE OF BLACK LEAF STREAK CONTROL IN ‘PISANG BERANGAN’ (AAA) IN PENINSULAR MALAYSIA

K.H. Then | S. Palaniappan

68. CR-M: AN IMPORTANT NUCLEOTIDE ACID SEQUENCE TO DISTINGUISH BANANA BUNCHY TOP VIRUS ISOLATES FROM TWO GROUPS

Y.H. Huang | J.W. Zeng | Y.L. Wu | R. Xia | G.J. Yi

69. INVESTIGATION INTO LOW-COST MEDIUM FOR HARDENING OF IN VITRO BANANA PLANTLETS TO PROMOTE ADOPTION OF DISEASE-FREE PLANTS

B. Jhurree-Dussoruth | H. Kallydin

70. MICROPROPAGATION OF ‘PISANG AWAK’ (MUSA, ABB GENOME) AS A MODEL FOR IMPROVING LIVELIHOODS OF RURAL COMMUNITIES IN MALAYSIA

L.K. Chan | V.H. Au | A.A.A. Noor | M. Adnan | P.L. Boey

71. HARNESSING THE POTENTIAL OF BANANA AND PLANTAIN IN ASIA AND THE PACIFIC FOR INCLUSIVE GROWTH

H.P. Singh

72. OPPORTUNITIES FOR BRIDGING THE GAP BETWEEN GENOMICS AND GENETIC IMPROVEMENT IN MUSA SPP.

N. Roux | M. Rouard | X.L. Huang | M. Smith

73. FUSARIUM WILT: THE BANANA DISEASE THAT REFUSES TO GO AWAY

R.C. Ploetz | A.C.L. Churchill