Articles
Investigating the role of strigolactones in control of shoot branching in cultivars of Zantedeschia
Article number
1104_25
Pages
165 – 170
Language
English
Abstract
Shoot branching is one of the major factors that influences crop productivity.
Different factors affect shoot branching, among which endogenous plant hormones are major controlling signals.
Recently, a new class of bioactive compounds, the strigolactones (SLs), has been found to be important in controlling branching.
The endogenous concentration of SLs was investigated in two Zantedeschia cultivars differing in their natural degree of branching.
A germination assay based on a parasitic weed Orobanche minor, which requires SLs for germination, was applied for analysis of SLs in guttation fluid.
A difference in concentration of SLs between these two cultivars was observed at an early stage of the annual growth cycle, supporting the hypothesis that SLs reduce shoot branching.
To test the hypothesis further, different highly branched selections of Zantedeschia, and the cultivar LSQUOGoldilocksRSQUO were treated with synthetic SL (GR24) via the growing medium during tissue culture.
GR24 reduced the number of axillary shoots but did not influence adventitious shoot production, providing evidence of SLs being an important candidate for controlling shoots originated from leaf axils in Zantedeschia.
Different factors affect shoot branching, among which endogenous plant hormones are major controlling signals.
Recently, a new class of bioactive compounds, the strigolactones (SLs), has been found to be important in controlling branching.
The endogenous concentration of SLs was investigated in two Zantedeschia cultivars differing in their natural degree of branching.
A germination assay based on a parasitic weed Orobanche minor, which requires SLs for germination, was applied for analysis of SLs in guttation fluid.
A difference in concentration of SLs between these two cultivars was observed at an early stage of the annual growth cycle, supporting the hypothesis that SLs reduce shoot branching.
To test the hypothesis further, different highly branched selections of Zantedeschia, and the cultivar LSQUOGoldilocksRSQUO were treated with synthetic SL (GR24) via the growing medium during tissue culture.
GR24 reduced the number of axillary shoots but did not influence adventitious shoot production, providing evidence of SLs being an important candidate for controlling shoots originated from leaf axils in Zantedeschia.
Authors
S. Manandhar, D.J. Woolley, K.A. Funnell
Keywords
branching inhibition, hormones, calla, in vitro, germination assay
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