In vitro regeneration of Exacum trinervium

Exacum trinervium was regenerated through axillary bud proliferation, direct organogenesis without intervening callus phase or indirect organogenesis through callus from leaf explants.
Leaf explants cultured on MS medium with 1 mg L^-1 BAP produced 12.66±2.00 shoots per explants while calli initiated on MS with 2 mg L^-1 2,4 D produced average of 6.33±1.26 shoots after transferring to MS medium with 1 or 2 mg L^-1 BAP. Nodal segments of field grown plants (11.00±4.5 shoots/node) and in vitro regenerated shoots (8.00±5.00 shoots/node) could be multiplied on MS medium with 2 mg L^-1 BAP. Rooting was achieved by transferring shoots regenerated from nodes and leaf explants on to MS with 1 or 2 mg L^-1 IBA. Better rooting response (88±2%) and higher number (11.00±2.5) of longer (7.47±2.7 cm) roots were observed at 2 mg L^-1 IBA. In vitro rooted plantlets were successfully (98%) acclimatized inside culture jars by placing sterilized gravel, coir dust and sand (1:1:1 ratio) as 1-cm layers.
Acclimatized plantlets were maintained on a potting medium consisting only of red soil until flowering inside a green house.

XXIX International Horticultural Congress on Horticulture: Sustaining Lives, Livelihoods and Landscapes (IHC2014): International Symposium on Ornamental Horticulture in the Global Greenhouse

S. Tennakoon, S.E. Peiris, J.P. Eeswara

rapid multiplication, Exacum

https://doi.org/10.17660/ActaHortic.2015.1104.36