Articles
Quantitative proteomic investigation on the effect of 1-methylcyclopropene treatments on postharvest quality of selected cut flowers
Article number
1104_47
Pages
311 – 318
Language
English
Abstract
Vase life or longevity of cut flowers is one of the important quality indices that determine the consumer preference and satisfaction.
In this study, we evaluated the effect of 1-methyl-cyclopropene (1-MCP) on postharvest quality of selected cut flowers with different ethylene sensitivities, and applied quantitative proteomic tools to reveal the significantly changed proteins.
Rose (Rosa hybrida), gerbera (Gerbera jamesonii H.), carnation (Dianthus caryophyllus) and snapdragon (Antirrhinum majus) were obtained from a local commercial grower and then divided into two groups.
One group was exposed to 0.9 µL L-1 1-MCP at 20°C for 16 h, while the other group served as control without 1-MCP treatment.
During a period of 14 days storage at 20°C, flower samples were taken after 1, 4, 7 and 14 days for quality evaluation. 1-MCP treatment significantly delayed the senescence of cut flowers and reduced wilting and abscission, especially for rose, gerbera and carnation flowers.
The correlation between chlorophyll fluorescence (flower sepals) changes and senescence of cut flowers was also determined.
By employing dimethylation labeling as a quantitative proteomic tool, more than 900 proteins were identified and quantified in rose flowers.
Among them, 103 proteins were found to represent five significant clusters based on quantitative protein changes.
Proteins involved in plant growth regulators, natural resistance and program cell death were increased during senescence, while those involved in protein metabolism, antioxidant and redox and methionine synthesis were decreased.
This study provides new insights on the regulation of senescence and effect of 1-MCP on quality of cut flowers, which may lay the foundation for better quality management.
In this study, we evaluated the effect of 1-methyl-cyclopropene (1-MCP) on postharvest quality of selected cut flowers with different ethylene sensitivities, and applied quantitative proteomic tools to reveal the significantly changed proteins.
Rose (Rosa hybrida), gerbera (Gerbera jamesonii H.), carnation (Dianthus caryophyllus) and snapdragon (Antirrhinum majus) were obtained from a local commercial grower and then divided into two groups.
One group was exposed to 0.9 µL L-1 1-MCP at 20°C for 16 h, while the other group served as control without 1-MCP treatment.
During a period of 14 days storage at 20°C, flower samples were taken after 1, 4, 7 and 14 days for quality evaluation. 1-MCP treatment significantly delayed the senescence of cut flowers and reduced wilting and abscission, especially for rose, gerbera and carnation flowers.
The correlation between chlorophyll fluorescence (flower sepals) changes and senescence of cut flowers was also determined.
By employing dimethylation labeling as a quantitative proteomic tool, more than 900 proteins were identified and quantified in rose flowers.
Among them, 103 proteins were found to represent five significant clusters based on quantitative protein changes.
Proteins involved in plant growth regulators, natural resistance and program cell death were increased during senescence, while those involved in protein metabolism, antioxidant and redox and methionine synthesis were decreased.
This study provides new insights on the regulation of senescence and effect of 1-MCP on quality of cut flowers, which may lay the foundation for better quality management.
Authors
J. Song, L. Fan, T. Hughes, L. Palmer Campbell, L. Li, X.H. Li
Keywords
rose (Rosa hybrida), gerbera (Gerbera jamesonii), carnation (Dianthus caryophyllus), snapdragon (Antirrhinum majus), chlorophyll fluorescence, quality, OFFgel, proteomics
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