Articles
Comparative RNA-seq for the investigation of tolerance to Verticillium wilt in black raspberry
Article number
1133_16
Pages
103 – 114
Language
English
Abstract
Verticillium dahliae Kleb., a cause of Verticillium wilt, is a wide-spread, soil-borne fungal pathogen with a wide host range that includes many fruit and vegetable crops. Verticillium dahliae has been isolated from Rubus species showing symptoms of the disease.
However, very little is known about the interactions of V. dahliae and woody plants in general, and Rubus in particular.
We have compared the root transcriptome sequence data of black raspberry (Rubus occidentalis L.) ‘Jewel’ inoculated with V. dahliae with that of uninfected ‘Jewel’ plants grown under greenhouse conditions.
From this analysis, 147 genes were found to be differentially expressed (DE). Eight of these genes had apparent homology to known or candidate genes involved in disease resistance.
Fourteen genes with homology to transcription factors were among the DE genes.
However, very little is known about the interactions of V. dahliae and woody plants in general, and Rubus in particular.
We have compared the root transcriptome sequence data of black raspberry (Rubus occidentalis L.) ‘Jewel’ inoculated with V. dahliae with that of uninfected ‘Jewel’ plants grown under greenhouse conditions.
From this analysis, 147 genes were found to be differentially expressed (DE). Eight of these genes had apparent homology to known or candidate genes involved in disease resistance.
Fourteen genes with homology to transcription factors were among the DE genes.
Publication
Authors
J.M. Bushakra, N.V. Bassil, J.E. Weiland, C.E. Finn, K.J. Vining, S. Filichkin, M. Dossett, D.W. Bryant, T.C. Mocker
Keywords
Rubus occidentalis, Verticillium dahliae, gene expression, disease resistance, marker development
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