Articles
Efficient adventitious shoot organogenesis on root explants of Ocimum basilicum L.
Article number
1187_10
Pages
89 – 92
Language
English
Abstract
Adventitious shoot regeneration efficiency of three cultivars of basil (Ocimum basilicum L.): ‘Grand Vert’, ‘Fin Vert’ and ‘Grand Vert Sweet’ was investigated in order to develop a mutagenesis and transformation system.
Isolated roots from in vitro seedlings were cut into explants of 10-15 mm and cultured in petri dishes containing 25 mL Murashige and Skoog medium (MS) supplemented with sucrose (30 g) and agar-agar (7 g) at pH 5,8. Two different cytokinins were tested individually: N6-Benzyladenine (BA: 0, 1, 5 or 10 μM) and thidiazuron (TDZ: 0, 1, 5 or 10 μM). The maximum percentage of explants showing regeneration (100%) was observed with the cultivar ‘Grand Vert’ on the medium containing 5 or 10 µM TDZ, in contrast to 10 µM BA that only induced 30% regeneration.
Each root explant produced one or more clusters of green nodule-like shoot meristems.
When the explants were transferred to MS medium without plant growth regulators, the shoots started to elongate.
This is the first report on shoot regeneration on root explants of basil.
Isolated roots from in vitro seedlings were cut into explants of 10-15 mm and cultured in petri dishes containing 25 mL Murashige and Skoog medium (MS) supplemented with sucrose (30 g) and agar-agar (7 g) at pH 5,8. Two different cytokinins were tested individually: N6-Benzyladenine (BA: 0, 1, 5 or 10 μM) and thidiazuron (TDZ: 0, 1, 5 or 10 μM). The maximum percentage of explants showing regeneration (100%) was observed with the cultivar ‘Grand Vert’ on the medium containing 5 or 10 µM TDZ, in contrast to 10 µM BA that only induced 30% regeneration.
Each root explant produced one or more clusters of green nodule-like shoot meristems.
When the explants were transferred to MS medium without plant growth regulators, the shoots started to elongate.
This is the first report on shoot regeneration on root explants of basil.
Authors
H. Fraj, C. Hannachi, S.P.O. Werbrouck
Keywords
basil (Ocimum basilicum L.), root, shoot organogenesis, 6-benzylaminopurine (BA), thidiazuron (TDZ)
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