Articles
Mycorrhizal synthesis between Lactarius deliciosus and Arbutus unedo under nursery conditions
Article number
1187_27
Pages
265 – 272
Language
English
Abstract
The use of strawberry tree plants artificially inoculated with mycorrhizal fungi allows the installation of more productive orchards benefiting from particular advantages conferred by the introduced fungi as, in the case of Lactarius deliciosus, the production of edible mushrooms.
In our previous work the compatibility between micropropagated Arbutus unedo plants and two isolates of Lactarius deliciosus was confirmed in vitro using synthesis tubes filled with a mixture of sterilized peat, vermiculite and perlite imbibed with nutrient solution.
The effect of inocula type and fungal isolate on the success of mycorrhiza establishment was analyzed.
Mycorrhizae synthesized in vitro persisted nine months after plants acclimatization and maintenance under nursery conditions.
In this work the viability of direct inoculation under nursery conditions was evaluated using seedlings germinated in unsterilized substrate.
Plants were inoculated with four L. deliciosus isolates multiplied in solid substrate.
Inoculation success was evaluated after 12 months.
All the mycorrhizal plants were installed on orchards with different edaphoclimatic conditions and will be monitored during 4 to 10 years, to confirm long term persistence of mycorrhizae and evaluate the fungal colonization level required to guarantee mushroom production.
In our previous work the compatibility between micropropagated Arbutus unedo plants and two isolates of Lactarius deliciosus was confirmed in vitro using synthesis tubes filled with a mixture of sterilized peat, vermiculite and perlite imbibed with nutrient solution.
The effect of inocula type and fungal isolate on the success of mycorrhiza establishment was analyzed.
Mycorrhizae synthesized in vitro persisted nine months after plants acclimatization and maintenance under nursery conditions.
In this work the viability of direct inoculation under nursery conditions was evaluated using seedlings germinated in unsterilized substrate.
Plants were inoculated with four L. deliciosus isolates multiplied in solid substrate.
Inoculation success was evaluated after 12 months.
All the mycorrhizal plants were installed on orchards with different edaphoclimatic conditions and will be monitored during 4 to 10 years, to confirm long term persistence of mycorrhizae and evaluate the fungal colonization level required to guarantee mushroom production.
Authors
H. Machado, M.J. Barrento, F. Plácito, D. Suárez, M. Clemente, P. Figueiredo, F. Gomes
Keywords
arbutoid mycorrhizae, strawberry tree, pure culture synthesis, nursery inoculation, ex vitro persistence
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