Articles
Magnification of the productivity of potato minituber seeds in tissue culture program using stem cutting
Article number
1187_28
Pages
273 – 284
Language
English
Abstract
Stem cuttings from potatoes are frequently used in multiplication program for disease-free seeds.
In vitro plantlets with stem cutting technique of three cultivars, i.e., ‘Diamant’, ‘Lady Balfour’ and ‘Red Baron’ were used as a good source material to develop tissue culture protocol of minitubers seed production in the greenhouse.
The first protocol (traditional) takes eight months to get microtubers (G0), including meristem tip culture, four subcultures, in vitro microtuberization, then seven months to produce minitubers (G1), including storage of microtubers, growth and harvesting, and eight months to produce the minitubers (G2) with total equals 23 months.
The second protocol (modified) takes ten months to get micro-minitubers (G0) through four in vitro subcultures and the plantlets were acclimatized and grown tell harvesting, and eight months to produce the minitubers (G1) with total equal of 18 months.
The third protocol (new procedure) designed to magnification of the productivity of potato minitubers seeds by increasing the number and reducing the time of its production during 2014-2015 seasons.
This protocol reduced the subcultures by two times then were acclimatized like the second protocol to produce micro-minitubers (G0) in eight months, then eight months to produce (G1) through a procedure using stem cuttings technique induced to grow at the leaf nodes by removal the apical growing point, with a total of 16 months.
The in vitro plantlets with stem cutting material source yielded significantly better seed potato micro-minitubers number/plant (217) than traditional (18) and modified protocols (8). Among the cultivars, ‘Diamant’ gave significantly a large minitubers number (123) per plant as compared to the other cultivars.
This indicates that in vitro plantlets with stem cutting technique have potential to give a viable material for seed potato minitubers production under greenhouses.
In addition, this production system shortens the time needed to get the number of minitubers with seven months than the traditional protocol.
Upon completion of the program via three ways to the second generation (G2) of minitubers seed, the production rate of the new procedure gave 48877 minitubers compared with 71 with the modified and 133 minitubers with the traditional protocols.
In vitro plantlets with stem cutting technique of three cultivars, i.e., ‘Diamant’, ‘Lady Balfour’ and ‘Red Baron’ were used as a good source material to develop tissue culture protocol of minitubers seed production in the greenhouse.
The first protocol (traditional) takes eight months to get microtubers (G0), including meristem tip culture, four subcultures, in vitro microtuberization, then seven months to produce minitubers (G1), including storage of microtubers, growth and harvesting, and eight months to produce the minitubers (G2) with total equals 23 months.
The second protocol (modified) takes ten months to get micro-minitubers (G0) through four in vitro subcultures and the plantlets were acclimatized and grown tell harvesting, and eight months to produce the minitubers (G1) with total equal of 18 months.
The third protocol (new procedure) designed to magnification of the productivity of potato minitubers seeds by increasing the number and reducing the time of its production during 2014-2015 seasons.
This protocol reduced the subcultures by two times then were acclimatized like the second protocol to produce micro-minitubers (G0) in eight months, then eight months to produce (G1) through a procedure using stem cuttings technique induced to grow at the leaf nodes by removal the apical growing point, with a total of 16 months.
The in vitro plantlets with stem cutting material source yielded significantly better seed potato micro-minitubers number/plant (217) than traditional (18) and modified protocols (8). Among the cultivars, ‘Diamant’ gave significantly a large minitubers number (123) per plant as compared to the other cultivars.
This indicates that in vitro plantlets with stem cutting technique have potential to give a viable material for seed potato minitubers production under greenhouses.
In addition, this production system shortens the time needed to get the number of minitubers with seven months than the traditional protocol.
Upon completion of the program via three ways to the second generation (G2) of minitubers seed, the production rate of the new procedure gave 48877 minitubers compared with 71 with the modified and 133 minitubers with the traditional protocols.
Authors
A.S. Ezzat, M.E. El-Denary
Keywords
potato, tissue culture, stem cutting, minitubers, seeds, magnification
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