Embryo rescue for breeding of early peach cultivar ‘Flored’ and determination of Sharka resistant genotypes with marker-assisted selection

Plum pox virus (PPV) causing Sharka disease is the most important viralagent for stone fruits.
This disease is most harmful to apricot, plum and peach trees.
This study aimed to obtain resistant peach genotypes to ‘Sharka’ disease with cross breeding method.
In this study, commercial peach cultivar ‘Flored’ with foreign apricot cultivar ‘Stark Early Orange’, and P 1908 (peach clone from Prunus davidiana) known to be resistant to PPV were crossed.
These crosses were germinated in liquid embryo rescue medium using MS, WPM and QL medium with different PGRs.
The best result was obtained from the one containing MS medium + BAP 1 mg L^-1 + GA₃ 0.1 mg L^-1 for germination percentage (% 87.5). A total of 59 peach genotypes from crossing among 2 combinations (FLxSEO, FLxP1908) were tested with SSR markers (PGS1.21, PGS1.24) and SSLP marker (ZP002). One genotype PPV resistance is expected to be candidate for PPV resistance in future studies.

IX International Symposium on In Vitro Culture and Horticultural Breeding

C. Unek, E. Tanrıver, A.B. Kuden

breeding, peach, apricot, cross-breeding, embryo rescue, marker selection

https://doi.org/10.17660/ActaHortic.2017.1187.29