Articles
In vitro propagation of nabq tree (Ziziphus spina-christi (L.) Desf.)
Article number
1187_8
Pages
69 – 78
Language
English
Abstract
Ziziphus spina-christi (L.) Desf. is a natural multipurpose plant belongs to family Rhamnaceae. Its ecological and economical values account for its great potential for the global market.
It is also one of the most drought and salinity tolerant fruit trees adapted to the ecological conditions of arid and semi-arid regions.
There is a need for applying non-conventional methods of propagation for conservation and sustainable utilization of the plant, therefore a study for creating an efficient micropropagation protocol was initiated.
Shoot tips and stem nodal segments from mature trees from Ras Sudr, South Sinai, were used as initial explants for in vitro propagation.
Autumn was the optimum time for explants collection.
Explants were cultured on solid Murashige and Skoog (MS) medium, and the influence of several plant growth regulators was investigated in order to ensure the best in vitro shoot initiation and multiplication.
MS medium without plant growth regulators was found to be highly suitable for shoots initiation and multiplication.
The effect of different types of auxins on the rooting of shoots was investigated.
MS medium containing 1.5 mg L-1 indole-3-butyric acid (IBA) gave the highest rooting percentage (66.7%) with the highest mean number and length of roots.
Effect of silver nitrate (AgNO3) at different concentrations in MS medium containing IBA at 1.5 mg L-1 on rooting improvement was evaluated.
The concentration of 2 mg L-1 AgNO3 significantly increased the rooting percentage, it reached the maximum value of 80%. A percentage of 66.7% of rooted plantlets were successfully acclimatized and grown normally in the greenhouse in a sterile soil mixture of sand, peat moss and vermiculite at equal volumes.
Hardened plantlets showed 100% survival outside the greenhouse.
It is also one of the most drought and salinity tolerant fruit trees adapted to the ecological conditions of arid and semi-arid regions.
There is a need for applying non-conventional methods of propagation for conservation and sustainable utilization of the plant, therefore a study for creating an efficient micropropagation protocol was initiated.
Shoot tips and stem nodal segments from mature trees from Ras Sudr, South Sinai, were used as initial explants for in vitro propagation.
Autumn was the optimum time for explants collection.
Explants were cultured on solid Murashige and Skoog (MS) medium, and the influence of several plant growth regulators was investigated in order to ensure the best in vitro shoot initiation and multiplication.
MS medium without plant growth regulators was found to be highly suitable for shoots initiation and multiplication.
The effect of different types of auxins on the rooting of shoots was investigated.
MS medium containing 1.5 mg L-1 indole-3-butyric acid (IBA) gave the highest rooting percentage (66.7%) with the highest mean number and length of roots.
Effect of silver nitrate (AgNO3) at different concentrations in MS medium containing IBA at 1.5 mg L-1 on rooting improvement was evaluated.
The concentration of 2 mg L-1 AgNO3 significantly increased the rooting percentage, it reached the maximum value of 80%. A percentage of 66.7% of rooted plantlets were successfully acclimatized and grown normally in the greenhouse in a sterile soil mixture of sand, peat moss and vermiculite at equal volumes.
Hardened plantlets showed 100% survival outside the greenhouse.
Authors
Gh.A. Hegazi, M.I. Diab, R.E. Abo El-Fadl
Keywords
Ziziphus, Rhamnaceae, micropropagation, shoot tips, stem nodal segments
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