Articles
Somatic embryogenesis in Phoenix dactylifera L. using floral bud explants
Article number
1187_2
Pages
13 – 28
Language
English
Abstract
Understanding the appropriate pathway of somatic embryogenesis in date palm (Phoenix dactylifera L.) would pave the way into conservation and commercialization of superior cultivars within a relatively short period.
In the current study, the floral buds were successfully utilized to propagate one of the most recalcitrant plant species via tissue culture.
Using floral buds as explants enabled us to overcome a set of in vitro obstacles facing the widely used shoot tip explants.
The immature intact inflorescence spathe, was excised from the parent tree and immersed in 30% sodium hypochlorite solution for 5 min.
The initial explants of floral bud spikelets induced yellowish compact callus after 2months on the starting medium containing 0.1 mg L-1 2,4 Di-chlorophenoxy acetic acid (2,4-D) and 1.0 mg L-1 2-isopentenyl adenine (2iP). Presence of 0.1 mg L-1 2.4-D in the medium was essential for the pro-embryos to undergo maturation and develop into white normal somatic embryos.
Histological study of explants indicated the influence of an accurate developmental stage of initial explants to respond to the culture medium and develop further into somatic embryos.
In the current study, the floral buds were successfully utilized to propagate one of the most recalcitrant plant species via tissue culture.
Using floral buds as explants enabled us to overcome a set of in vitro obstacles facing the widely used shoot tip explants.
The immature intact inflorescence spathe, was excised from the parent tree and immersed in 30% sodium hypochlorite solution for 5 min.
The initial explants of floral bud spikelets induced yellowish compact callus after 2months on the starting medium containing 0.1 mg L-1 2,4 Di-chlorophenoxy acetic acid (2,4-D) and 1.0 mg L-1 2-isopentenyl adenine (2iP). Presence of 0.1 mg L-1 2.4-D in the medium was essential for the pro-embryos to undergo maturation and develop into white normal somatic embryos.
Histological study of explants indicated the influence of an accurate developmental stage of initial explants to respond to the culture medium and develop further into somatic embryos.
Authors
A.A. Abul-Soad, K.S. Emara, A.S. Abdallah, S.M. Mahdi
Keywords
floral buds, callus, conservation, somatic embryogenesis, Phoenix dactylifera L., STDF
Online Articles (31)