Articles
In vitro establishment and multiplication of hardy kiwi (Actinidia arguta ‘Issai’)
Article number
1187_6
Pages
51 – 58
Language
English
Abstract
Plant tissue culture is a very efficient tool for plant propagation.
However, can be really complicated since different procedures have to be developed for different species and even different genotypes within the same species.
In this study, a new procedure for Actinidia arguta ‘Issai’ in vitro establishment and multiplication was investigated.
Different explant sterilization protocols and plant growth regulators combinations (1 mg L-1 BAP + 0.2 mg L-1 GA3 and 1 mg L-1 BAP + 0.1 mg L-1 NAA) were tested to avoid the high surface contamination detected and to establish the in vitro culture.
The best results for contamination (0%) and shoot survival (100%) were achieved when the surface of the plant explants were sterilized during 1 min in 70% EtOH followed by 20 min in 0.4% sodium hypochlorite with 4 drops of Tween® 20. Also the highest percentage of shoot induction (100%) was obtained when the basal media was supplemented with 1 mg L-1 BAP + 0.2 mg L-1 GA3. Finally, independently of the induction media used, the shoot proliferation average 3 when the basal medium was supplemented with 1 mg L-1 BAP + 1mg L-1 GA3. In conclusion, an effective protocol for in vitro establishment and multiplication of Actinidia arguta ‘Issai’ was successfully designed.
However, can be really complicated since different procedures have to be developed for different species and even different genotypes within the same species.
In this study, a new procedure for Actinidia arguta ‘Issai’ in vitro establishment and multiplication was investigated.
Different explant sterilization protocols and plant growth regulators combinations (1 mg L-1 BAP + 0.2 mg L-1 GA3 and 1 mg L-1 BAP + 0.1 mg L-1 NAA) were tested to avoid the high surface contamination detected and to establish the in vitro culture.
The best results for contamination (0%) and shoot survival (100%) were achieved when the surface of the plant explants were sterilized during 1 min in 70% EtOH followed by 20 min in 0.4% sodium hypochlorite with 4 drops of Tween® 20. Also the highest percentage of shoot induction (100%) was obtained when the basal media was supplemented with 1 mg L-1 BAP + 0.2 mg L-1 GA3. Finally, independently of the induction media used, the shoot proliferation average 3 when the basal medium was supplemented with 1 mg L-1 BAP + 1mg L-1 GA3. In conclusion, an effective protocol for in vitro establishment and multiplication of Actinidia arguta ‘Issai’ was successfully designed.
Authors
R. Hameg, P.P. Gallego, M.E. Barreal
Keywords
baby kiwi, plant tissue culture, benzylaminopurine BAP, gibberellic acid GA3, 1-naphthaleneacetic acid (NAA), contamination
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