Articles
Analysis on cloning and expression of CsAP1-2 gene in Cymbidium sinense
Article number
1414_1
Pages
1 – 14
Language
English
Abstract
Cymbidium sinense is one of the typical representatives of traditional orchids in China, with elegant and fragrant flowers, possessing high ornamental value. AP1 (APETALA1), as a class A gene of the MADS-box gene family, not only determines the floral transition, but also controls the development of sepals and petals.
In this study, an AP1 gene was cloned from the flower buds of C. sinense and named CsAP1-2. Sequencing revealed that CsAP1-2 isolated three gene fragments, which may be different alternative splicing, encoding 241 amino acids (CsAP1-2a), 172 amino acids (CsAP1-2b), and 162 amino acids (CsAP1-2c), respectively.
Phylogenetic analysis showed that CsAP1-2a, CsAP1-2b and CsAP1-2c were all categorized as class A genes of MADS-box.
In terms of protein structural domains, both CsAP1-2a and CsAP1-2c possessed conserved MADS structural domains and K structural domains, whereas CsAP1-2b possessed only MADS structural domains without K structural domains due to the deletion of the intermediate part of the fragment.
In addition, both CsAP1-2a and CsAP1-2b contain the FUL motif and paleoAP1 motif of the AP1/FUL (APETALA 1/FRUITFULL) subfamily, while CsAP1-2c does not contain the FUL motif and paleoAP1 motif due to the deletion of the C-terminal part.
Analysis of the spatiotemporal expression patterns showed that among the different organs (roots, stems, leaves, flowers and fruits), CsAP1-2 expression was highest in flowers, followed by stems.
During the different stages of flower development, CsAP1-2 was concentrated in the early stages of flower development, with the highest expression in S1, followed by a gradual decrease in expression, and the lowest expression in S5. The expression of CsAP1-2 was highest in the gynostemium and lowest in the sepals in different parts of the floral tissues, and the expression pattern of the two alternative splicing of CsAP1-2 was similar to that of the full-length sequence.
In this study, an AP1 gene was cloned from the flower buds of C. sinense and named CsAP1-2. Sequencing revealed that CsAP1-2 isolated three gene fragments, which may be different alternative splicing, encoding 241 amino acids (CsAP1-2a), 172 amino acids (CsAP1-2b), and 162 amino acids (CsAP1-2c), respectively.
Phylogenetic analysis showed that CsAP1-2a, CsAP1-2b and CsAP1-2c were all categorized as class A genes of MADS-box.
In terms of protein structural domains, both CsAP1-2a and CsAP1-2c possessed conserved MADS structural domains and K structural domains, whereas CsAP1-2b possessed only MADS structural domains without K structural domains due to the deletion of the intermediate part of the fragment.
In addition, both CsAP1-2a and CsAP1-2b contain the FUL motif and paleoAP1 motif of the AP1/FUL (APETALA 1/FRUITFULL) subfamily, while CsAP1-2c does not contain the FUL motif and paleoAP1 motif due to the deletion of the C-terminal part.
Analysis of the spatiotemporal expression patterns showed that among the different organs (roots, stems, leaves, flowers and fruits), CsAP1-2 expression was highest in flowers, followed by stems.
During the different stages of flower development, CsAP1-2 was concentrated in the early stages of flower development, with the highest expression in S1, followed by a gradual decrease in expression, and the lowest expression in S5. The expression of CsAP1-2 was highest in the gynostemium and lowest in the sepals in different parts of the floral tissues, and the expression pattern of the two alternative splicing of CsAP1-2 was similar to that of the full-length sequence.
Publication
Authors
J.C. Liu, R. Zhou, C.Q. Lu, F.X. Yang
Keywords
C. sinense, CsAP1-2 gene, bioinformatics, expression fraction, flowering organ development
Online Articles (11)