Articles
A key role of photomorphogenesis interaction network in leaf variegation of Phalaenopsis
Article number
1414_2
Pages
15 – 32
Language
English
Abstract
Phalaenopsis holds critical positions in horticultural plants because of its high ornamental and economic values.
At present, leaf variegation becomes an ever more important goal for breeding of Phalaenopsis. To understand the anatomical and genetic regulatory mechanism in leaf variegation, the ultra-structural character, physiological features and RNA-Seq based comparative transcriptomics of leaves on both wild type and leaf color mutants in Phalaenopsis were analyzed.
Chlorophyll and carotenoid contents of yellow sectors in leaf color mutant (Y) were significantly lower than those of wild type (WT) and green sectors in mutant (G). Chloroplasts with well-organized structure were found only in WT and GS but not in Y. Gene ontology analysis showed that most of the DEGs related to chloroplast and chlorophyll metabolism were downregulated in Y compared with WT and G. In KEGG pathways, pathway related to porphyrin and chlorophyll metabolism showed significant difference in Y compared to WT and G. Quantitative real-time PCR (RT-PCR) further confirmed the same trend and consistent results of eight genes between the RT-PCR and the transcriptome data.
Two interaction prediction networks were found in this research.
These two networks were related to photomorphogenesis and photosynthesis pigments biogenesis.
All protein-encoding genes involving these two networks were significantly downregulated in Y compared to WT and G. Conclusively, impaired photomorphogenesis led to the abnormity of chloroplast.
Abnormal chloroplast resulted in the decrease of chlorophyll and carotenoid contents which changed the leaf color from green to yellow.
At present, leaf variegation becomes an ever more important goal for breeding of Phalaenopsis. To understand the anatomical and genetic regulatory mechanism in leaf variegation, the ultra-structural character, physiological features and RNA-Seq based comparative transcriptomics of leaves on both wild type and leaf color mutants in Phalaenopsis were analyzed.
Chlorophyll and carotenoid contents of yellow sectors in leaf color mutant (Y) were significantly lower than those of wild type (WT) and green sectors in mutant (G). Chloroplasts with well-organized structure were found only in WT and GS but not in Y. Gene ontology analysis showed that most of the DEGs related to chloroplast and chlorophyll metabolism were downregulated in Y compared with WT and G. In KEGG pathways, pathway related to porphyrin and chlorophyll metabolism showed significant difference in Y compared to WT and G. Quantitative real-time PCR (RT-PCR) further confirmed the same trend and consistent results of eight genes between the RT-PCR and the transcriptome data.
Two interaction prediction networks were found in this research.
These two networks were related to photomorphogenesis and photosynthesis pigments biogenesis.
All protein-encoding genes involving these two networks were significantly downregulated in Y compared to WT and G. Conclusively, impaired photomorphogenesis led to the abnormity of chloroplast.
Abnormal chloroplast resulted in the decrease of chlorophyll and carotenoid contents which changed the leaf color from green to yellow.
This paper comes with a set of 3 supplementary tables and 4 supplementary figures.
Click here to download the .zip file containing the supplemental data.
Publication
Authors
J. Gao, F.X. Yang, D. Liang, Y.L. Wei, J.P. Jin, G.F. Zhu
Keywords
leaf variegation, Phalaenopsis, photomorphogenesis, interaction network
Online Articles (11)