Articles
Microsatellite fingerprinting and evaluation of genetic relationship for new perspective walnut genotypes from Nikita Botanical Gardens
Article number
1420_7
Pages
49 – 54
Language
English
Abstract
A study was conducted with the aim to investigate genetic polymorphism and relationship for a set of new promising walnut genotypes from the collection of genetic resources of Nikita Botanical Gardens (Yalta). Twelve WGA SSR-markers were used for the genotyping of 21 walnut accessions among which set of modern perspective genotypes are presented along with older Crimean cultivars.
A specific SSR-profile was established for all studied genotypes.
In total 71 alleles were identified for whole SSR-markers set.
The number of alleles for each locus ranged from two (WGA 79, WGA 4) to eight (WGA069, WGA276) with average about six alleles per locus.
According to the level of polymorphism of SSR loci it can be concluded there is a respectively high level of genetic diversity.
Cluster analysis revealed two main clusters, one of which contains cultivars, consisting of two groups.
Noteworthy is that one cultivar, which is characterized by a very late flowering period was allocated into the out group.
Relationship among more modern and older walnut cultivars and breeding selections is being discussed.
A specific SSR-profile was established for all studied genotypes.
In total 71 alleles were identified for whole SSR-markers set.
The number of alleles for each locus ranged from two (WGA 79, WGA 4) to eight (WGA069, WGA276) with average about six alleles per locus.
According to the level of polymorphism of SSR loci it can be concluded there is a respectively high level of genetic diversity.
Cluster analysis revealed two main clusters, one of which contains cultivars, consisting of two groups.
Noteworthy is that one cultivar, which is characterized by a very late flowering period was allocated into the out group.
Relationship among more modern and older walnut cultivars and breeding selections is being discussed.
Publication
Authors
S. Khokhlov, Yu. Pluganar, I. Suprun, S. Tokmakov
Keywords
Juglans regia, polymorphism, genotyping, molecular identification, genetic similarity
Groups involved
Online Articles (45)