Articles
Molecular characterization of plum pox virus isolates in apricot in Serbia
Article number
1450_32
Pages
249 – 256
Language
English
Abstract
Plum pox virus (PPV), a potyvirus from the family Potyviridae, is the most detrimental virus affecting stone fruit species, causing Sharka disease.
PPV can significantly decrease fruit quality and yield in sensitive cultivars.
It was reported for the first time in Serbia on plums in 1935. Out of the ten identified, only three major PPV strains (PPV-M, PPV-D, and PPV-Rec) are present in the country.
To explore the occurrence and presence of PPV strains in apricot in Serbia, field surveys were carried out from 2008 to 2022. Visual inspections indicated that a very low percentage of the trees exhibited the leaf symptoms of Sharka.
A total of 282 leaf samples were collected from different locations across the country.
These samples were analysed by immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) using strain-specific primers targeting two genomic regions (Nter CP and CI). PPV was confirmed in a single infection in 86 samples.
PPV-Rec was the most prevalent strain (52 samples), followed by PPV-M (21 samples) and PPV-D (13 samples). Mixed infection (PPV-Rec + PPV-D) was confirmed in six samples.
Although widely present in plums, PPV was less frequently detected in apricots in Serbia.
The presented results confirmed the wide distribution and dominant presence of PPV-Rec isolates in apricots in Serbia.
The genetic diversity of the PPV isolates was evaluated by sequencing a PCR fragment located in the Cter NIb-Nter CP (CP) and C-terminal part of P3, the full 6K1, and the N-terminal part of the CI (P3-6K1) coding regions for selected isolates.
PPV can significantly decrease fruit quality and yield in sensitive cultivars.
It was reported for the first time in Serbia on plums in 1935. Out of the ten identified, only three major PPV strains (PPV-M, PPV-D, and PPV-Rec) are present in the country.
To explore the occurrence and presence of PPV strains in apricot in Serbia, field surveys were carried out from 2008 to 2022. Visual inspections indicated that a very low percentage of the trees exhibited the leaf symptoms of Sharka.
A total of 282 leaf samples were collected from different locations across the country.
These samples were analysed by immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) using strain-specific primers targeting two genomic regions (Nter CP and CI). PPV was confirmed in a single infection in 86 samples.
PPV-Rec was the most prevalent strain (52 samples), followed by PPV-M (21 samples) and PPV-D (13 samples). Mixed infection (PPV-Rec + PPV-D) was confirmed in six samples.
Although widely present in plums, PPV was less frequently detected in apricots in Serbia.
The presented results confirmed the wide distribution and dominant presence of PPV-Rec isolates in apricots in Serbia.
The genetic diversity of the PPV isolates was evaluated by sequencing a PCR fragment located in the Cter NIb-Nter CP (CP) and C-terminal part of P3, the full 6K1, and the N-terminal part of the CI (P3-6K1) coding regions for selected isolates.
Publication
Authors
D. Jevremović, B. Vasilijević, V. Katanić, B. Popović
Keywords
Prunus armeniaca L., plum pox virus, IC-RT-PCR, PPV-Rec
Online Articles (59)