Articles
IN VITRO SHOOT REGENERATION FROM INTERNODE SEGMENTS AND INTERNODE – DERIVED CALLUS OF BLUEBERRY (VACCINIUM SPP.)
Article number
346_17
Pages
125 – 130
Language
Abstract
A protocol was developed to regenerate viable shoots from internode stem segments in blueberry.
This protocol is currently being used to induce somaclonal variation in blueberry with the objective of selecting for high pH tolerance.
Optimum regeneration occurred when either of the top 2 (youngest) internodes were selected as explants, rather than older internode stem segments (3 through 7) for two cytokinins studied.
Explants were cultured 6 weeks on Zimmerman’s Z-2 medium supplemented with 25 uM zeatin or 25 uM thidiazuron.
Explant response to the treatments varied and included: no response, callus growth only, callus growth and subsequent shoot formation originating from the callus mass, and adventitious shoot formation directly from the internode segment without an intervening callus.
In general, greatest shoot regeneration was obtained on medium supplemented with 25 uM zeatin.
This protocol is currently being used to induce somaclonal variation in blueberry with the objective of selecting for high pH tolerance.
Optimum regeneration occurred when either of the top 2 (youngest) internodes were selected as explants, rather than older internode stem segments (3 through 7) for two cytokinins studied.
Explants were cultured 6 weeks on Zimmerman’s Z-2 medium supplemented with 25 uM zeatin or 25 uM thidiazuron.
Explant response to the treatments varied and included: no response, callus growth only, callus growth and subsequent shoot formation originating from the callus mass, and adventitious shoot formation directly from the internode segment without an intervening callus.
In general, greatest shoot regeneration was obtained on medium supplemented with 25 uM zeatin.
Publication
Authors
James D. Hruskoci, Paul E. Read
Keywords
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