Articles
IN VITRO PROPAGATION OF THIN-LEAF SUNFLOWER (HELIANTHUS DECAPETALUS L.)
Article number
397_11
Pages
125 – 128
Language
Abstract
In vitro propagation of thin-leaf sunflower (Helianthus decapetalus L.) was achieved by subculturing shoot sections with nodes in Murashige-Skoog (MS) medium supplemented with 0.1 mg N6-benzyladenine (BA) · liter-1. A multiplication rate of 4.9 per 22-day culture period was achieved, resulting in theoretically yielding 110.4 billion shoots from a shoot tip in a year.
Shoots elongated from these in vitro cultured nodes could be rooted in MS medium with 0.1 mg indole-3-butyric acid (IBA) · liter-1. Plants produced were readily acclimatized and flowered in a greenhouse.
Shoots elongated from these in vitro cultured nodes could be rooted in MS medium with 0.1 mg indole-3-butyric acid (IBA) · liter-1. Plants produced were readily acclimatized and flowered in a greenhouse.
Authors
T. Hosoki, K. Ohta, K. Inaba, M. Harisaki
Keywords
Micropropagation, Node-culture, Perennial-sunflower, BA (N6-benzyladenin), IBA (indole-3-butyric acid)
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