TYPING KIWIFRUIT CULTIVARS BY MEANS OF RAPD MARKERS

Eighty 10-base long arbitrary primers were tested for PCR-based DNA amplification in Actinidia. Twenty primers were selected from a first screening.
They gave two to eight polymorphic fragments in 13 kiwifruit cultivars.
The cultivars assayed were the three males (‘Matua’, ‘M3h’, and ‘Tomuri’) and ten females (‘Abbott’, ‘Allison’, Blake’, ‘Bruno’, ‘Constricted’, ‘Elmwood’, ‘Gracie’, ‘Hayward’, ‘Monty’, and ‘Top Star’®). All kiwifruit cultivars were separated each from any other, excepting ‘Abbott’, ‘Hayward’ and ‘Top Star’®

The Stoffel fragment was found suitable for RAPD analysis producing on average 19% more bands per primer and 40% more polymorphisms per primer than did Taq polymerase.
The reproducibility of the results, which is the most severe drawback of RAPD technique, can be improved by repeating at least twice each amplification and by disregarding any bands that were not well scorable.

III International Symposium on Kiwifruit

G. Cipriani, R. Di Bella, R. Testolin

Actinidia, breeding, DNA fingerprinting, polymerase chain reaction, Stoffel fragment

https://doi.org/10.17660/ActaHortic.1997.444.13