Articles
MICROPROPAGATION, PROTOPLAST CULTURE AND ITS IMPLICATIONS IN THE IMPROVEMENT OF SCENTED ROSE
Article number
547_18
Pages
147 – 158
Language
Abstract
Damask rose, Rosa damascena, an important species among the scented roses, yields a highly fragrant commercially important essential oil.
It is commonly used in perfumery industry, beverages, soft drinks, medicines etc.
Besides rose oil, the other products are rose water, rose concrete, rose absolute, “gulkand” (a sugary preperation) etc. R. bourboniana, a related species, is also used also used for rose oil extraction.
For achieving faster rates of multiplication, tissue culture methods are best employed and may be of great commercial value in establishing plantations.
Micropropagation protocols using nodal segments were established in R. damascena and R. bourboniana. Rooted plants were transferred to field.
In addition protoplast culture studies were also carried out in the two species of scented rose.
Friable callus was initiated from stem and leaf segments inoculated on Murashige and Skoog (1962) medium supplemented with varying concentrations of 2,4-D (1-10 µM), NAA (1-10 µM), BAP (1-10 µM). Efficient protoplast culture procedures have been worked out from cell suspension as a source, however, regeneration of plants posed a developmental block in both parental and hybrid calli.
Alternative explants for their regeneration potential are under assessment and promising results are obtained for genetic manipulation of these important essential oil bearing rose species.
It is commonly used in perfumery industry, beverages, soft drinks, medicines etc.
Besides rose oil, the other products are rose water, rose concrete, rose absolute, “gulkand” (a sugary preperation) etc. R. bourboniana, a related species, is also used also used for rose oil extraction.
For achieving faster rates of multiplication, tissue culture methods are best employed and may be of great commercial value in establishing plantations.
Micropropagation protocols using nodal segments were established in R. damascena and R. bourboniana. Rooted plants were transferred to field.
In addition protoplast culture studies were also carried out in the two species of scented rose.
Friable callus was initiated from stem and leaf segments inoculated on Murashige and Skoog (1962) medium supplemented with varying concentrations of 2,4-D (1-10 µM), NAA (1-10 µM), BAP (1-10 µM). Efficient protoplast culture procedures have been worked out from cell suspension as a source, however, regeneration of plants posed a developmental block in both parental and hybrid calli.
Alternative explants for their regeneration potential are under assessment and promising results are obtained for genetic manipulation of these important essential oil bearing rose species.
Authors
P.K. Pati, M. Sharma, P.S. Ahuja
Keywords
Rosa damascena, Rosa bourboniana, Damask rose, in vitro multiplication, microshoots, rooting, hardening, direct regeneration, protoplast fusion.
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