Articles
PROPAGATION OF GROUND COVER ROSES BY STEM CUTTINGS AND TISSUE CULTURE
Article number
547_45
Pages
371 – 376
Language
Abstract
The effect of propagation time on rooting of the softwood cuttings of ground cover roses (eight cultivars) was evaluated in 1997-1999. The cuttings were taken in June and August from mother plants grown on the rootstock in the field.
Next they were placed in the greenhouse in temperature 230C in the media: peat + perlite (1:1 v/v). The percentage of rooted cuttings and their quality depends on tested cultivars and for most of them was significantly better in June than in August.
The best rooting range occurred to following cultivars: ‘Alba Meidiland’, ‘Ferdy’, ‘Red Fairy’, ‘The Fairy’ and ‘Sommermorgen’ (from 92- 100%). The cuttings of ‘Mainaufeuer’ rooted in the lowest percentage in both data: in June – 79,4%, in August -34,4 %.
The in vitro shoot cultures were established and multiplied on a modified Murashige and Skoog (1962) medium.
The multiplication rate, rooting and acclimatisation ability of the shoots were found out.
The effect of the in vitro atmosphere enrichment with 0,2% CO2 and rooting methods on the quality of microcuttings and their acclimatisation in rockwool in a greenhouse was examined.
The highest multiplication rate (over 5) and 100% rooting and acclimatisation was noted for ‘ Heidetraum’, also for the direct shoots rooting in a greenhouse.
Microcuttings of all other cultivars required the in vitro root induction treatment for 8-9 days.
The treatment with CO2 markedly enhanced the shoot quality and effectiveness of acclimatisation.
Next they were placed in the greenhouse in temperature 230C in the media: peat + perlite (1:1 v/v). The percentage of rooted cuttings and their quality depends on tested cultivars and for most of them was significantly better in June than in August.
The best rooting range occurred to following cultivars: ‘Alba Meidiland’, ‘Ferdy’, ‘Red Fairy’, ‘The Fairy’ and ‘Sommermorgen’ (from 92- 100%). The cuttings of ‘Mainaufeuer’ rooted in the lowest percentage in both data: in June – 79,4%, in August -34,4 %.
The in vitro shoot cultures were established and multiplied on a modified Murashige and Skoog (1962) medium.
The multiplication rate, rooting and acclimatisation ability of the shoots were found out.
The effect of the in vitro atmosphere enrichment with 0,2% CO2 and rooting methods on the quality of microcuttings and their acclimatisation in rockwool in a greenhouse was examined.
The highest multiplication rate (over 5) and 100% rooting and acclimatisation was noted for ‘ Heidetraum’, also for the direct shoots rooting in a greenhouse.
Microcuttings of all other cultivars required the in vitro root induction treatment for 8-9 days.
The treatment with CO2 markedly enhanced the shoot quality and effectiveness of acclimatisation.
Authors
H. Wisniewska-Grzeszkiewicz, M. Podwyszynska
Keywords
roses, propagation time, softwood cuttings, tissue culture, CO2 , multiplication rate
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