Articles
PHYLOGENETIC ANALYSES OF GENUS ROSA: POLYPHYLY OF SECTION PIMPINELLIFOLIAE AND ORIGIN OF ROSA X FORTUNIANA LINDL
Article number
547_43
Pages
357 – 363
Language
Abstract
We investigated whether the genus Rosa, section Pimpinellifoliae is polyphyletic or not based on matK sequence variability.
We sequenced the matK region of four species in the section Pimpinellifoliae, Rosa forrestiana in the section Rosa and conducted a phylogenetic analysis with 27 rose species and Agrimonia pilosa, which were sequenced previously (Matsumoto et al., 1998). The four species with three species previously sequenced in the section Pimpinellifoliae were devided into three groups.
The species in the three groups were closely related both phenetically and cladistically.
Rosa x fortuniana is thought to be a hybrid between R. laevigata and R. banksiae. To confirm the origin of Rosa x fortuniana, we determined its nrDNA ITS and chloroplast matK sequences.
The ITS was composed of two types of sequences, one a complete match with the sequence of R. banksiae, the other a match with R. laevigata except for one nucleotide.
Moreover, the matK sequence was matched with R. laevigata, not R. banksiae. From the results, it was confirmed that R. x fortuniana is a hybrid between a seed parent, R. laevigata, and a pollen parent, R. banksiae. As R. x fortuniana was first produced more than 150 years ago, this approach might be useful in investigating the hybrid origin of other old roses.
We sequenced the matK region of four species in the section Pimpinellifoliae, Rosa forrestiana in the section Rosa and conducted a phylogenetic analysis with 27 rose species and Agrimonia pilosa, which were sequenced previously (Matsumoto et al., 1998). The four species with three species previously sequenced in the section Pimpinellifoliae were devided into three groups.
The species in the three groups were closely related both phenetically and cladistically.
Rosa x fortuniana is thought to be a hybrid between R. laevigata and R. banksiae. To confirm the origin of Rosa x fortuniana, we determined its nrDNA ITS and chloroplast matK sequences.
The ITS was composed of two types of sequences, one a complete match with the sequence of R. banksiae, the other a match with R. laevigata except for one nucleotide.
Moreover, the matK sequence was matched with R. laevigata, not R. banksiae. From the results, it was confirmed that R. x fortuniana is a hybrid between a seed parent, R. laevigata, and a pollen parent, R. banksiae. As R. x fortuniana was first produced more than 150 years ago, this approach might be useful in investigating the hybrid origin of other old roses.
Authors
S. Matsumoto, H. Nishio, Y. Ueda, H. Fukui
Keywords
chloroplast DNA, ITS, matK, PCR, phylogeny, rose
Online Articles (53)