Articles
PARTIAL CHARACTERIZATION OF TWO POTYVIRUSES ASSOCIATED WITH GOLDEN SPIDER LILY SEVERE MOSAIC DISEASE
Article number
568_18
Pages
127 – 134
Language
English
Abstract
Golden spider lily (Lycoris aurea Herb.), a species native to Taiwan, is currently a popular cut flower ornamental bulb crop in Taiwan.
Foliar mild mottling has been found in spider lily nurseries for many years.
In 1995, some spider lily plants exhibiting severe mosaic were detected.
Inoculum prepared from severe mosaic tissue was able to induce local lesions on Chenopodium quinoa whereas inoculum prepared from mild mottle tissue failed to infect C. quinoa. Tests with 25 known potyvirus antisera failed to detect the presence of viruses in infected spider lilies by ELISA. The antigens, however, reacted with potyvirus-specific monoclonal antibodies.
Electron microscopy revealed the presence of flexuous rod-shaped virus particles and pinwheel inclusions in leaf dips and ultra-thin sections of infected severe mosaic tissues, respectively.
Purification resulted in typical potyvirus particles measuring about 750 nm.
Two species of protein with estimated relative mass of 35 and 37 kDa were detected from samples purified from tissues bearing severe mosaic symptoms.
An antiserum (065) was subsequently produced.
Following the same purification procedure, only a 37 kDa species protein was detected in the sample purified from mild mottle tissue.
In SDS-immunodiffusion test, antiserum 065 reacted with its homologous antigen prepared from severe mosaic tissues and produced two precipitation bands.
It also reacted with the antigen extracted from mild mottle tissue by producing only one precipitation band.
The results indicate that the severe mosaic disease of golden spider lily is caused by two serologically distinct potyviruses and that one, provisionally named Lycoris mild mottle virus, induces mild mottle symptoms.
The other potyvirus, capable of inducing local lesions on C. quinoa, is tentatively named Lycoris potyvirus.
Foliar mild mottling has been found in spider lily nurseries for many years.
In 1995, some spider lily plants exhibiting severe mosaic were detected.
Inoculum prepared from severe mosaic tissue was able to induce local lesions on Chenopodium quinoa whereas inoculum prepared from mild mottle tissue failed to infect C. quinoa. Tests with 25 known potyvirus antisera failed to detect the presence of viruses in infected spider lilies by ELISA. The antigens, however, reacted with potyvirus-specific monoclonal antibodies.
Electron microscopy revealed the presence of flexuous rod-shaped virus particles and pinwheel inclusions in leaf dips and ultra-thin sections of infected severe mosaic tissues, respectively.
Purification resulted in typical potyvirus particles measuring about 750 nm.
Two species of protein with estimated relative mass of 35 and 37 kDa were detected from samples purified from tissues bearing severe mosaic symptoms.
An antiserum (065) was subsequently produced.
Following the same purification procedure, only a 37 kDa species protein was detected in the sample purified from mild mottle tissue.
In SDS-immunodiffusion test, antiserum 065 reacted with its homologous antigen prepared from severe mosaic tissues and produced two precipitation bands.
It also reacted with the antigen extracted from mild mottle tissue by producing only one precipitation band.
The results indicate that the severe mosaic disease of golden spider lily is caused by two serologically distinct potyviruses and that one, provisionally named Lycoris mild mottle virus, induces mild mottle symptoms.
The other potyvirus, capable of inducing local lesions on C. quinoa, is tentatively named Lycoris potyvirus.
Authors
C.-A. Chang, C.-C. Chen, H.-T. Hsu
Keywords
electron microscopy, inclusion bodies, Lycoris aurea, Lycoris mild mottle virus, Lycoris viruses, serology
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