Articles
PHYTOPLASMA DETECTION IN SOME ORNAMENTAL PLANTS PROPAGATED IN VITRO
Article number
568_35
Pages
237 – 245
Language
English
Abstract
The results of PCR examination indicated that during two years of tissue culture at continuous temperature of 20oC phytoplasmas could be detected in diseased plants of annual statice kept on the medium with and without plant regulators.
Detectability of phytoplasmas in tissues of infected strawflower and gladiolus was relatively lower than in annual statice.
In the case of strawflower it was limited to shoots micropropagated on the medium with BAP or without hormones and in the case of gladiolus – to shoots propagated in vitro on medium with BAP. Neither pathological changes nor phytoplasmas were detected by electron microscopy of thin sections of plantlets derived from either diseased or healthy plants, after 24 months in vitro.
To test whether phytoplasmas are sensitive to temperature, phytoplasma affected micropropagated gladiolus plants were grown on various media and at the reduced temperatures of 4 or 13oC. PCR analysis indicated that phytoplasma detection was more successful after the storage at low temperature.
Plants stored from one to three months at the reduced temperature tended to have higher titre of phytoplasma than the plants maintained at continuous (20oC) temperature.
Relatively, the best detection was observed for plants on the medium containing BAP+NAA.
Detectability of phytoplasmas in tissues of infected strawflower and gladiolus was relatively lower than in annual statice.
In the case of strawflower it was limited to shoots micropropagated on the medium with BAP or without hormones and in the case of gladiolus – to shoots propagated in vitro on medium with BAP. Neither pathological changes nor phytoplasmas were detected by electron microscopy of thin sections of plantlets derived from either diseased or healthy plants, after 24 months in vitro.
To test whether phytoplasmas are sensitive to temperature, phytoplasma affected micropropagated gladiolus plants were grown on various media and at the reduced temperatures of 4 or 13oC. PCR analysis indicated that phytoplasma detection was more successful after the storage at low temperature.
Plants stored from one to three months at the reduced temperature tended to have higher titre of phytoplasma than the plants maintained at continuous (20oC) temperature.
Relatively, the best detection was observed for plants on the medium containing BAP+NAA.
Authors
M. Kaminska, E. Gabryszewska, M. Korbin, A. Rudzinska-langwald
Keywords
Limonium, Helichrysum, Gladiolus, micropropagation, phytoplasma
Online Articles (37)