Articles
A FABAVIRUS INDUCING RINGSPOT DISEASE IN LISIANTHUS
Article number
568_5
Pages
51 – 57
Language
English
Abstract
A virus was isolated from lisianthus (Eustoma russellianum (Don.) Griseb) showing virus-like disease symptoms.
In lisianthus, symptoms began on lower leaves with yellow spots and an irregular concentric ring pattern of dark green and pale green tissues which eventually developed into necrotic concentric rings.
In addition to these symptoms, mottling, necrotic local lesions, and, sometimes, malformation were observed on leaves of other susceptible plants.
Examination of crude extracts of infected lisianthus leaves by electron microscopy revealed the presence of spherical particles measuring 28 nm in diameter when stained with 2% uranyl acetate.
Similar particles were present in cytoplasm but not in nuclei in ultrathin sections of leaf tissues from virus-infected lisianthus, Nicotiana rustica, and Petunia hybrida. Cytoplasmic lattice structures and amorphous inclusions containing virions were also observed in ultrathin sections of leaf tissues of infected Chenopodium quinoa.
Differential centrifugation followed by sucrose density gradient centrifugation of extracts of infected C. quinoa or N. rustica yielded virion particles about 28 nm in diameter.
Analysis by SDS-polyacrylamide gel electrophoresis of purified virus preparations showed that virions contained two structural polypeptides about 24 and 43 kD relative molecular weight.
Tests by ELISA indicate that the virus is a member of the genus Fabavirus.
In lisianthus, symptoms began on lower leaves with yellow spots and an irregular concentric ring pattern of dark green and pale green tissues which eventually developed into necrotic concentric rings.
In addition to these symptoms, mottling, necrotic local lesions, and, sometimes, malformation were observed on leaves of other susceptible plants.
Examination of crude extracts of infected lisianthus leaves by electron microscopy revealed the presence of spherical particles measuring 28 nm in diameter when stained with 2% uranyl acetate.
Similar particles were present in cytoplasm but not in nuclei in ultrathin sections of leaf tissues from virus-infected lisianthus, Nicotiana rustica, and Petunia hybrida. Cytoplasmic lattice structures and amorphous inclusions containing virions were also observed in ultrathin sections of leaf tissues of infected Chenopodium quinoa.
Differential centrifugation followed by sucrose density gradient centrifugation of extracts of infected C. quinoa or N. rustica yielded virion particles about 28 nm in diameter.
Analysis by SDS-polyacrylamide gel electrophoresis of purified virus preparations showed that virions contained two structural polypeptides about 24 and 43 kD relative molecular weight.
Tests by ELISA indicate that the virus is a member of the genus Fabavirus.
Authors
C.C. Chen, C.C. Hu, Y.K. Chen, H.T. Hsu
Keywords
Broad bean wilt virus, electron microscopy, Eustoma russellianum, Fabavirus, lisianthus virus, Myzus persicae, serology
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