Articles
DETECTION AND IDENTIFICATION OF POTYVIRUSES IN ZANTEDESCHIA
Article number
568_20
Pages
143 – 148
Language
English
Abstract
In a survey 4 Zantedeschia species and c. 30 hybrids were tested in DAS-ELISA and ACP-ELISA with DsMV-antiserum and two potyvirus-specific monoclonal antibodies (PTY 1 and AS-0228), respectively.
Dasheen mosaic virus (DsMV), which has been reported earlier as the most prevalent virus in aroids, including Zantedeschia, was detected in only a limited amount of samples.
Therefore potyviruses in Zantedeschia plants from different origins were further characterized by RT-PCR and sequence analysis.
Four different potyviruses could be distinguished, viz.
DsMV, konjac mosaic virus (KoMV), bean yellow mosaic virus and an hitherto unidentified potyvirus.
In 50 samples tested with a virus specific RT-PCR this potyvirus and KoMV appeared to be more prevalent than DsMV.
In Zantedeschia potyviruses are not equally distributed over the various parts of the plant either in single or mixed infections.
Virus indexing was most reliable in the second half of the growing season when testing the middle or inner leaves.
The four potyviruses can be detected by PTY 1 and AS-0228 antisera showing that the PTY 1 serum is the most sensitive serum.
Dasheen mosaic virus (DsMV), which has been reported earlier as the most prevalent virus in aroids, including Zantedeschia, was detected in only a limited amount of samples.
Therefore potyviruses in Zantedeschia plants from different origins were further characterized by RT-PCR and sequence analysis.
Four different potyviruses could be distinguished, viz.
DsMV, konjac mosaic virus (KoMV), bean yellow mosaic virus and an hitherto unidentified potyvirus.
In 50 samples tested with a virus specific RT-PCR this potyvirus and KoMV appeared to be more prevalent than DsMV.
In Zantedeschia potyviruses are not equally distributed over the various parts of the plant either in single or mixed infections.
Virus indexing was most reliable in the second half of the growing season when testing the middle or inner leaves.
The four potyviruses can be detected by PTY 1 and AS-0228 antisera showing that the PTY 1 serum is the most sensitive serum.
Authors
K. Pham, S.A. Langeveld, M.E.C. Lemmers, A.F.L.M. Derks
Keywords
dasheen mosaic virus, konjac mosaic virus, bean yellow mosaic virus, RT-PCR, serology, sequences
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