Articles
COLLECTION OF ORNAMENTAL PLANT VIRUSES
Article number
568_28
Pages
193 – 199
Language
English
Abstract
At Pruhonice the research on virus diseases has a long tradition.
Twenty six viruses were isolated and identified by serology, host range and electron microscopy.
Isolates of these viruses in leaf material were dried and stored over calcium chloride at 4 °C. More than 50 isolates of 16 viruses economically important in ornamental crops grown in this country are maintained in a collection to serve as reference virus material (Arabis mosaic virus, Bean yellow mosaic virus, Chrysanthemum virus B, Cucumber mosaic virus, Cymbidium mosaic virus, Dasheen mosaic virus, Impatiens necrotic spot virus, Odontoglossum ring spot virus, Pelargonium flower break virus, Poplar mosaic virus, Potato virus Y, Tobacco mosaic virus, Tomato aspermy virus, Tomato mosaic virus, Tomato spotted wilt virus and Tulip breaking virus). Some isolates (especially those of Tospoviruses) are kept also in living plants.
Antisera to most of the viruses in the collection are available.
They were obtained from other virological laboratories or from commercial firms (Loewe Germany, Bioreba Switzerland and Sanofi France).
The collection of ornamental plant viruses was included into the Czech National Programme on Protection of Genetic Resources of Economically Significant Microorganisms and Tiny Animals.
The Programme is composed of 15 different collections centred in 9 institutions at 15 laboratories.
The research, collecting, evidence, long-term maintenance and exploiting of the collections is coordinated by the Research Institute of Crop Production – Division of Phytomedicine.
Twenty six viruses were isolated and identified by serology, host range and electron microscopy.
Isolates of these viruses in leaf material were dried and stored over calcium chloride at 4 °C. More than 50 isolates of 16 viruses economically important in ornamental crops grown in this country are maintained in a collection to serve as reference virus material (Arabis mosaic virus, Bean yellow mosaic virus, Chrysanthemum virus B, Cucumber mosaic virus, Cymbidium mosaic virus, Dasheen mosaic virus, Impatiens necrotic spot virus, Odontoglossum ring spot virus, Pelargonium flower break virus, Poplar mosaic virus, Potato virus Y, Tobacco mosaic virus, Tomato aspermy virus, Tomato mosaic virus, Tomato spotted wilt virus and Tulip breaking virus). Some isolates (especially those of Tospoviruses) are kept also in living plants.
Antisera to most of the viruses in the collection are available.
They were obtained from other virological laboratories or from commercial firms (Loewe Germany, Bioreba Switzerland and Sanofi France).
The collection of ornamental plant viruses was included into the Czech National Programme on Protection of Genetic Resources of Economically Significant Microorganisms and Tiny Animals.
The Programme is composed of 15 different collections centred in 9 institutions at 15 laboratories.
The research, collecting, evidence, long-term maintenance and exploiting of the collections is coordinated by the Research Institute of Crop Production – Division of Phytomedicine.
Authors
V. Mokra, B. Gotzova, J. Mertelik, J. Polak
Keywords
long-term maintenance of plant viruses, desiccation over calcium chloride, Czech Republic
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