CIS-REGULATORY ELEMENTS FOR PISTIL SPECIFIC EXPRESSION IN S-RNASE PROMOTER REGION OF JAPANESE PEAR (PYRUS PYRIFOLIA NAKAI)

The pistil-specific and temporal expression of Japanese pear S-RNase gene is regulated by S-RNase promoter region.
To identify the cis-regulatory elements on the S-RNase promoter of Japanese pear, we analysed GUS activities in transgenic tobacco plants carrying 9 sequential deletions of S₃-RNase promoter fused to the -glucuronidase (GUS) gene.
GUS activity was detected quantitatively in the pistils from the transgenic plants carrying the promoter deleted from -747 to -310. GUS activity was localized in the stigmatic secretory zone, and the transmitting tissues in the pistils but not in other tissues; pollen, anthers, petals, sepals, leaves and roots.
GUS activities in the pistils increased steadily from 4 days before flowering.
No transgenic pistils with deletions carrying -255 or shorter promoters displayed GUS activity, indicating that the critical cis-regulatory elements for pistil specific expression were present between -310 and -255 of S₃-RNase promoter region.
Furthermore, deletions from -552 to -356 led to the drastic decrease of GUS activity in the transgenic pistil, which assumes that enhancer-like element for the high level of expression in pistils are present within this region.

International Symposium on Asian Pears, Commemorating the 100th Anniversary of Nijisseiki Pear

D.M.R.K.K. Dissanayake, S. Norioka, N. Norioka, T. Takasaki, T. Nakanishi

Pyrus pyrifolia, self-incompatibility, deletion promoter, GUS, transgenic plant

https://doi.org/10.17660/ActaHortic.2002.587.60