Articles
PEACH BREEDING PROGRAMME FOR EARLY RIPENING, LOW CHILLING REQUIREMENT CULTIVARS: EMBRYO RESCUE AND SOMATIC EMBRYOGENESIS
Article number
592_16
Pages
125 – 129
Language
English
Abstract
The typical climatic characteristics of the Sicilian areas, together with the local genetic pool available, allow peach [Prunus persica (L.) Batsch.] production for more than seven months (mid April to end November). For the late ripening season, peach production is based on the local germplasm, but for the early ripening season there are no native varieties available.
Therefore, a breeding program has been undertaken.
One of the main goals of this breeding programme is widening the varietal range of the early ripening cultivars characterized by low chilling requirement.
To reach this objective, genotypes with these characters are used as parents in crosses.
In vitro embryo rescue is necessary when the seed parent is an early ripening cultivar.
Success of immature embryo culture depends on several factors, particularly plant genotype, embryo development stage and media composition.
In this study, the effects of mineral salt composition, carbon source and growth regulators in the medium and the effect of genotype on the in vitro germination of immature Flordastar embryos were investigated.
Direct and indirect somatic embryogenesis were also observed from immature embryos obtained from the Armking x Flordastar crosses.
Secondary embryogenesis was obtained as well.
Therefore, a breeding program has been undertaken.
One of the main goals of this breeding programme is widening the varietal range of the early ripening cultivars characterized by low chilling requirement.
To reach this objective, genotypes with these characters are used as parents in crosses.
In vitro embryo rescue is necessary when the seed parent is an early ripening cultivar.
Success of immature embryo culture depends on several factors, particularly plant genotype, embryo development stage and media composition.
In this study, the effects of mineral salt composition, carbon source and growth regulators in the medium and the effect of genotype on the in vitro germination of immature Flordastar embryos were investigated.
Direct and indirect somatic embryogenesis were also observed from immature embryos obtained from the Armking x Flordastar crosses.
Secondary embryogenesis was obtained as well.
Publication
Authors
M.L. Mancuso, T. Caruso, M.A. Germanà
Keywords
peach, embryo rescue, somatic embryogenesis
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