Articles
CHARACTERIZATION AND EXPRESSION OF TWO GENES ENCODING ETHYLENE RECEPTORS IN PEACH FRUIT
Article number
592_79
Pages
583 – 588
Language
English
Abstract
We have isolated two peach (Prunus persica) genes, Pp-ETR1 and Pp-ERS1, homolog to the Arabidopsis ethylene receptor ETR1 and ERS1. Pp-ETR1 is identical, in terms of exons number and introns position, to At-ETR1 although the first and fifth intron are 5 and 20 times longer, respectively.
In addition two putative polyadenilation sites, that may cause an incomplete splicing at the 3′ terminus, are present into the fifth intron.
Translation of such a truncated transcript would lead to a product missing a large portion of the receiver domain.
The coding region of Pp-ERS1 is organized in five exons interrupted by four introns, but unlike Pp-ETR1 no marked differences in terms of intron length between At-ERS1 and Pp-ERS1 have been detected.
Into the promoter region of Pp-ERS1 a motif of 28 nt, which shows high homology with binding ethylene-factors detected in genes up-regulated by ethylene, is present.
The deduced protein of both genes contain a sensor domain and the Histidine kinase domain, in which residues, thought to be important for the normal function of ETR1 and ERS-type proteins as ethylene receptors are conserved.
These results indicate that Pp-ETR1 and Pp-ERS1 could be functional ethylene receptors with the ability to bind ethylene.
Expression analysis, carried out by quantitative RT-PCR, was performed during fruit ripening (cv Maria Marta). The level of Pp-ETR1 transcripts remained unchanged throughout ripening, whereas Pp-ERS1 mRNA increased in parallel with the ethylene climacteric.
In addition two putative polyadenilation sites, that may cause an incomplete splicing at the 3′ terminus, are present into the fifth intron.
Translation of such a truncated transcript would lead to a product missing a large portion of the receiver domain.
The coding region of Pp-ERS1 is organized in five exons interrupted by four introns, but unlike Pp-ETR1 no marked differences in terms of intron length between At-ERS1 and Pp-ERS1 have been detected.
Into the promoter region of Pp-ERS1 a motif of 28 nt, which shows high homology with binding ethylene-factors detected in genes up-regulated by ethylene, is present.
The deduced protein of both genes contain a sensor domain and the Histidine kinase domain, in which residues, thought to be important for the normal function of ETR1 and ERS-type proteins as ethylene receptors are conserved.
These results indicate that Pp-ETR1 and Pp-ERS1 could be functional ethylene receptors with the ability to bind ethylene.
Expression analysis, carried out by quantitative RT-PCR, was performed during fruit ripening (cv Maria Marta). The level of Pp-ETR1 transcripts remained unchanged throughout ripening, whereas Pp-ERS1 mRNA increased in parallel with the ethylene climacteric.
Publication
Authors
C. Bonghi, A. Rasori, F. Ziliotto, A. Ramina, P. Tonutti
Keywords
quantitative RT-PCR, ETR1, ERS1, Ethylene Binding protein, Prunus persica
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