Articles
BRASSINOSTEROID BIOSYNTHESIS INHIBITION AS A SIDE EFFECT OF THE COMMONLY USED IMIDAZOLE FUNGICIDE IMAZALIL
Article number
636_25
Pages
205 – 210
Language
English
Abstract
Dark germinating Arabidopsis thaliana seedlings showed a clearly de-etiolated phenotype on a medium with 2.5 μM imazalil (IMA). Comparable with brassinosteroid deficient mutants, hypocotyl length was reduced and cotyledons had opened.
A major part of the hypocotyl length reduction could be restored by 0.1 μM epibrasinolide (EBR). Combining EBR with 2.5 μM GA3 was necessary for a complete restoration of the hypocotyl length.
The dose dependent root length reduction by IMA could not be overcome by adding EBR. These experiments suggest that one of the side effects of IMA is the inhibition of the brassinosteroid biosynthesis.
A major part of the hypocotyl length reduction could be restored by 0.1 μM epibrasinolide (EBR). Combining EBR with 2.5 μM GA3 was necessary for a complete restoration of the hypocotyl length.
The dose dependent root length reduction by IMA could not be overcome by adding EBR. These experiments suggest that one of the side effects of IMA is the inhibition of the brassinosteroid biosynthesis.
Authors
S.P.O. Werbrouck, P.C. Debergh
Keywords
Arabidopsis, brassinolide, de-etiolation, gibberellic acid
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