Articles
CHANGES OF JASMONIC ACID DURING RIPENING IN PEAR FRUIT AND INTERACTIONS BETWEEN JASMONIC ACID AND ABSCISIC ACID
Article number
636_68
Pages
537 – 543
Language
English
Abstract
Changes of jasmonic acid (JA) during ripening in La France pear fruit (Pyrus communis L.), and interactions between JA and abscisic acid (ABA) were investigated.
Endogenous JA and ABA were analyzed using gas chromatography-mass spectrometry (GC-MS). Interactions between JA and ABA were examined using fruit discs.
Although JA increased rapidly after harvest [165 days after full bloom (DAFB)], it did not coincide with the changes of internal ethylene concentrations.
Since ethylene production in the discs was not induced by the JA treatment, the increase of JA after harvest may have been caused by water loss from the fruit after harvest.
The discs from pulp excised 160 DAFB (preclimacteric) and 174 DAFB (9 days in storage; climacteric) were collected and placed in petri dishes including a 0.4 mol mannitol solution with a combination of JA, ABA and aminoethoxyvinylglycine (AVG). JA and ABA concentrations in the discs were measured at 0, 24, 48 and 72 hr after the initiation of treatment.
At the preclimacteric stage, JA or JA plus AVG treatment decreased endogenous ABA concentrations.
This result suggests that JA may influence endogenous ABA synthesis separately from ethylene.
At the climacteric stage, the ABA treatment increased endogenous JA concentrations and decreased it when combined with AVG. This result also demonstrates that ABA may influence endogenous JA synthesis via ethylene.
Endogenous JA and ABA were analyzed using gas chromatography-mass spectrometry (GC-MS). Interactions between JA and ABA were examined using fruit discs.
Although JA increased rapidly after harvest [165 days after full bloom (DAFB)], it did not coincide with the changes of internal ethylene concentrations.
Since ethylene production in the discs was not induced by the JA treatment, the increase of JA after harvest may have been caused by water loss from the fruit after harvest.
The discs from pulp excised 160 DAFB (preclimacteric) and 174 DAFB (9 days in storage; climacteric) were collected and placed in petri dishes including a 0.4 mol mannitol solution with a combination of JA, ABA and aminoethoxyvinylglycine (AVG). JA and ABA concentrations in the discs were measured at 0, 24, 48 and 72 hr after the initiation of treatment.
At the preclimacteric stage, JA or JA plus AVG treatment decreased endogenous ABA concentrations.
This result suggests that JA may influence endogenous ABA synthesis separately from ethylene.
At the climacteric stage, the ABA treatment increased endogenous JA concentrations and decreased it when combined with AVG. This result also demonstrates that ABA may influence endogenous JA synthesis via ethylene.
Authors
S. Kondo, H. Seto
Keywords
aminoethoxyvinylglycine, ethylene, fruit discs, Pyrus communis L.
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