AN APPROACH TO THE REGULATION OF THE IN VITRO FLOWERING OF ROOT EXPLANTS OF CICHORIUM INTYBUS L.

The first experiments on in vitro flowering were made in 1961 by Chouard and Aghion (1), who were using cultures of flower stems of Nicotiana tabacum L., cultivar "Wisconsin 38", this variety being indifferent to photoperiodic and cold treatments.

In 1964, Paulet and Nitsch (2,3) obtained in vitro flowers on root explants of Cichorium intybus L., a species which needs cold and photoperiodic treatments in order to flower.
The in vitro flowering of chicory was also studied by Margara (4), while Wellensiek (5) worked on the entire root.
Later on, Nitsch and Nitsch (6) obtained flowers on the stem explants of a short day plant, Plumbago indica.
But in all these experiments, only the primary cultures flowered, not the second pricking out.

Recently, the laboratory of USSR academician Tchailakhyan (Constantinova and al. (7) obtained flowers in vitro with fragments of stems of Nicotiana tabacum, cultivar Trapezond, after three passages on the medium.

International Symposium on Flower Formation in Ornamentals

P. Paulet

https://doi.org/10.17660/ActaHortic.1977.68.22