Articles
PATHOGENICITY, MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF RHIZOCTONIA SPP. ISOLATES FROM STRAWBERRY PLANTS IN ISRAEL
Article number
708_25
Pages
155 – 160
Language
English
Abstract
Methyl-bromide is currently used as a soil fumigant for strawberry.
However, its use is restricted internationally and in Israel from 2005. Thus, soil borne patho-gens including Rhizoctonia spp., may cause more serious losses than previously re-ported.
It is therefore important to study Rhizoctonia populations on strawberry in Israel.
Anastomosis grouping (AG), the method currently used to classify Rhizoctonia isolates, is accurate and widely used; however, it is time and labour consuming.
In addition, certain isolates do not self anastomose, or anastomose with more than one group.
In the present work, rDNA-ITS sequence multiple alignment was used to clas-sify the isolates.
The isolates were consistently clustered according to their AGs.
Cur-rently, determination of pathogenicity of Rhizoctonia spp. isolates on strawberry is based on reduction of plant biomass rather than on specific disease symptoms.
The duration of this test is relatively time-consuming (3-5 weeks). Based on inoculation of strawberry petioles, fruit and young seedlings derived from strawberry seeds, inocu-lation of seedlings at the two-true-leaf stage was found to be an adequate method for determining pathogenicity of Rhizoctonia isolates, and is less time-consuming than the method currently used.
However, its use is restricted internationally and in Israel from 2005. Thus, soil borne patho-gens including Rhizoctonia spp., may cause more serious losses than previously re-ported.
It is therefore important to study Rhizoctonia populations on strawberry in Israel.
Anastomosis grouping (AG), the method currently used to classify Rhizoctonia isolates, is accurate and widely used; however, it is time and labour consuming.
In addition, certain isolates do not self anastomose, or anastomose with more than one group.
In the present work, rDNA-ITS sequence multiple alignment was used to clas-sify the isolates.
The isolates were consistently clustered according to their AGs.
Cur-rently, determination of pathogenicity of Rhizoctonia spp. isolates on strawberry is based on reduction of plant biomass rather than on specific disease symptoms.
The duration of this test is relatively time-consuming (3-5 weeks). Based on inoculation of strawberry petioles, fruit and young seedlings derived from strawberry seeds, inocu-lation of seedlings at the two-true-leaf stage was found to be an adequate method for determining pathogenicity of Rhizoctonia isolates, and is less time-consuming than the method currently used.
Publication
Authors
M. Sharon, B. Sneh, S. Freeman
Keywords
Rhizoctonia, Fragaria x ananassa, pathogenicity, rDNA-ITS
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