Home > V International Strawberry Symposium > DEVELOPMENT OF PCR-RFLP MARKER ON STRAWBERRY AND THE IDENTIFICATION OF CULTIVARS AND THEIR PROGENY

Articles

DEVELOPMENT OF PCR-RFLP MARKER ON STRAWBERRY AND THE IDENTIFICATION OF CULTIVARS AND THEIR PROGENY

Article number

708_91

Pages

517 – 522

Language

English

Abstract

We developed 24 PCR-RFLP markers for strawberry (Fragaria × ananassa Duch.) cultivar identification.
We used these co-dominant markers to successfully distinguish 65 commercial cultivars.
The markers were also capable of distinguishing closely related strains (96 progeny of a selfed Sachinoka line). The results were highly reproducible across DNA extraction methods, organs, and researchers.
The stability and simplicity of the PCR-RFLP analysis makes it a valuable tool for identifying strawberry cultivars.
In addition, we investigated the inheritance of these markers using selfed lines of Sachinoka, Tochihime, Nyoho, and Cesena, and checked whether it was consistent with Mendelian law.

Publication

V International Strawberry Symposium

Authors

M. Kunihisa, N. Fukino, S. Matsumoto

Keywords

Cultivar identification, PCR-RFLP marker, reproducibility, selfed-line, co-dominance, Mendelian inheritance

Full text

https://doi.org/10.17660/ActaHortic.2006.708.91

Online Articles (108)

1. ROOT GROWTH AND STRUCTURE IN STRAWBERRY AS AF-FECTED BY ORGANIC RESIDUES

D. Neri | G. Savini

2. THE OPTIMUM TEMPERATURE FOR LONG-SEASON CROPPING IN THE EVERBEARING STRAWBERRY ´EVEREST´

A. Wagstaffe | N.H. Battey

3. POLYETHYLENE MULCH, DEFICIT IRRIGATION, OVERHEAD SPRINKLING AND STRAWBERRY FRUIT BRONZING

K.D. Larson | S.T. Koike | F.G. Zalom

4. IMPACT OF PRODUCTION SYSTEMS AND FERTILIZER APPLI-CATION ON YIELD AND QUALITY OF STRAWBERRIES

M. Martinsson | A. Kwast | G. Cieslinski | W. Treder

5. WATER CHANNELS IN STRAWBERRY, AND THEIR ROLE IN THE PLANT¿S RESPONSE TO WATER STRESS

M.M. Blanke | D.T. Cooke

6. SUSTAINABILITY OF COLD-CLIMATE STRAWBERRY PRODUCTION SYSTEMS

M.D. Stevens | B.L. Black | J.D. Lea-Cox | C.J. Hapeman

7. ADAPTATION AND AGRONOMICAL CHARACTERIZATION OF ´MEDINA´ AND ´MARINA´ STRAWBERRY CULTIVARS

8. MISSHAPED FRUIT IN STRAWBERRY, AN AGRONOMIC EVALUATION

J. Lopez-Medina | A. Palacio-Villegas | E. Vazquez-Ortiz

9. SUPPRESSION OF AGPASE IN TRANSGENIC STRAWBERRY PLANTS ABOLISHES THE LEAF STARCH DIURNAL CYCLE, BUT HAS NO EFFECT ON NORMAL VEGETATIVE AND REPRODUCTIVE DEVELOPMENT

10. EFFECTS OF PLANT GROWTH REGULATORS ON OUT-OF-SEASON PRODUCTION OF STRAWBERRY

M. Hamano | H. Yamazaki | S. Imada

11. THE EFFECT OF PLANTING SYSTEM ON THE YIELD OF STRAWBERRY GROWN OUT-OF-SEASON

B. Duralija | Z. Cmelik | J. Druzic Orlic | T. Milicevic

12. NITROGEN REQUIREMENTS OF DRIP IRRIGATED STRAWBERRIES GROWN IN SUBTROPICAL ENVIRONMENTS

13. CALCIUM THIOSULFATE DOES NOT INFLUENCE YIELD, POSTHARVEST QUALITY, OR CALCIUM CONTENT OF ´SWEET CHARLIE´ STRAWBERRY

C.E. Esmel | J.R. Duval | S.A. Sargent

14. PEST MANAGEMENT IN QUEENSLAND STRAWBERRIES: REALITY BITES, AND GROWERS´ PERSPECTIVES CHANGE!

G.K. Waite

15. GETTING A GRIP ON THRIPS IN STRAWBERRIES

M.Y. Steiner | S. Goodwin

16. SYMPATRIC SUBPOPULATIONS OF BOTRYTIS CINEREA ON STRAWBERRIES BASED ON THE CONTENT OF TRANSPOSABLE ELEMENTS AND THEIR CONNECTION WITH RESISTANCE TO BOTRYTICIDES

T. Milicevic | S. Topolovec-Pintaric | B. Cvjetkovic | D. Ivic | B. Duralija

17. SCREENING FOR STRAWBERRY PLANT RESISTANCE TO PHYTOPHTHORA CACTORUM IN A NUTRIENT FILM TECHNIQUE (NFT) SYSTEM

P. Parikka

18. RESISTANCE OF SELECTED STRAWBERRY CULTIVARS TO ANTHRACNOSE FRUIT ROT AND BOTRYTIS FRUIT ROT

C.K. Chandler | J.C. Mertely | N. Peres

19. THE SENSITIVITY OF STRAWBERRY CULTIVARS TO MAJOR FUNGAL FOLIAR DISEASES IN CROATIA, AND THE POSSIBILITIES FOR THEIR CONTROL

T. Milicevic | S. Topolovec-Pintaric | B. Cvjetkovic | B. Duralija

20. SURVIVAL OF COLLETOTRICHUM ACUTATUM IN DEAD PLANT MATERIAL AND SOIL IN FINLAND

P. Parikka | A. Lemmetty | E. Pääskynkivi

21. THE EFFECT OF FOLIAR-APPLIED CA AND SI ON THE SEVERITY OF POWDERY MILDEW IN TWO STRAWBERRY CULTIVARS

S. Palmer | E. Scott | J. Stangoulis | A.J. Able

22. OCCURRENCE AND IDENTIFICATION OF ASTER YELLOWS RELATED PHYTOPLASMA IN STRAWBERRY IN POLAND AND LITHUANIA

M. Cieslinska | B. Komorowska | J. Stankiene

23. EFFECT OF PREHARVEST CALCIUM APPLICATION ON GREY MOULD DEVELOPMENT AND POSTHARVEST QUALITY IN STRAWBERRIES

M. Naradisorn | A. Klieber | M. Sedgley | E. Scott | A.J. Able

24. THIRTY YEARS OF ADVANCES IN ARTHROPOD MANAGEMENT IN FLORIDA´S COMMERCIAL STRAWBERRIES

J.F. Price | C.K. Chandler | J.R. Duval | S.I. Rondon | D. Cantliffe

25. PATHOGENICITY, MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF RHIZOCTONIA SPP. ISOLATES FROM STRAWBERRY PLANTS IN ISRAEL

M. Sharon | B. Sneh | S. Freeman

26. PHYTOPLASMA SUB-GROUPS INFECTING INSECTS COLLECTED IN DAMAGED STRAWBERRY FIELDS

M. Pastore | S. Paltrinieri | A. Bertaccini | R. Priore | V. Graziano

27. MAIN STRAWBERRY DISEASES IN IRAPUATO, GUANAJUATO AND ZAMORA, MICHOACAN, MEXICO

J. Narro-Sanchez | P.A. Davalos-Gonzalez | R. Velasquez-Valle | J. Castro-Franco

28. RESISTANCE OF SELECTED STRAWBERRY CULTIVARS TO ROOT-KNOT NEMATODE SPECIES (MELOIDOGYNE SPP.)

J.A. Cobon | J. Trott

29. IMPACT OF GLOBAL METHYL BROMIDE PHASE-OUT ON THE SUSTAINABILITY OF STRAWBERRY INDUSTRIES

L.J. Porter | S.W. Mattner | J. Banks | P. Fraser

30. STRAWBERRY NURSERIES: SUMMARIES OF ALTERNATIVES AND TRIALS IN DIFFERENT GEOGRAPHIC REGIONS

I.J. Porter | S.W. Mattner | R.C. Mann | R.K. Gounder

31. EFFECT OF BIOFUMIGATION ON TYPICAL WEEDS OF STRAWBERRY FIELDS

32. ETHANEDINITRILE (C₂N₂) – A NOVEL SOIL FUMIGANT FOR STRAWBERRY PRODUCTION

33. SEVEN YEARS´ WORK ON ALTERNATIVES TO METHYL BROMIDE (MB) FOR STRAWBERRY PRODUCTION IN HUELVA (SPAIN)

34. STRAWBERRY TRANSPLANT PRODUCTION AND PERFORMANCE IN ANNUAL PLASTICULTURE SYSTEM IN THE EASTERN UNITED STATES

F. Takeda | P. Perkins-Veazie | H.J. Swartz | S. Hokanson

35. THE PERFORMANCE OF STRAWBERRY PLUGS IN QUEENSLAND

M.C. Menzel | G.K. Waite

36. NORTHERN VIGOUR® IN STRAWBERRY CROWNS

37. EVALUATION OF TRAY-PLANT QUALITY IN THE PROPAGATION PHASE

S. Gianluca | A. Letouzé | C. Sabbadini | D. Neri

38. MERISTEM ANALYSIS ON STRAWBERRY PLANTS DURING PROPAGATION AND PRODUCTION

39. INFLUENCE OF MICROPROPAGATION ON THE PRODUCTION OF STRAWBERRY RUNNER PLANTS, YIELD AND QUALITY

A. Kikas | A. Libek | V. Vasar

40. EVALUATION OF STRAWBERRY NURSERY MANAGEMENT TECHNIQUES TO IMPROVE QUALITY OF PLANTS

R. Pertuzé | M. Barrueto | V. Diaz | M. Gamardella

41. STRAWBERRY RUNNER SUPPRESSION WITH PROHEXADIONE-CALCIUM

B.L. Black

42. RUNNER CERTIFICATION AND VIRUS ELIMINATION IN COMMERCIAL STRAWBERRY CULTIVARS IN AUSTRALIA

R. Lines | G. Kelly | M. Milinkovic | B. Rodoni

43. HOT WATER TREATMENT TO ELIMINATE COLLETOTRICHUM ACUTATUM FROM STRAWBERRY RUNNER CUTTINGS

A.W. Johnson | D.W. Simpson | A. Berrie

44. EVALUATION OF PHYTOSANITARY STATUS OF COLD-STORED RUNNER PLANTS OF STRAWBERRY IN BASILICATA, SOUTHERN ITALY

I. Camele | A. Caponera | M. Cirulli | F. Marras | S. Frisullo

45. CRYOPRESERVATION OF IN VITRO CULTURED SHOOT TIPS OF STRAWBERRY

M. Zhao | Z. Wang | J. Su | Y. Qian

46. EFFECT OF MICRO-PROPAGATION ON THE HEALTH STATUS OF STRAWBERRY PLANTING MATERIAL FOR COMMERCIAL PRODUCTION OF STRAWBERRY RUNNERS FOR QUEENSLAND

J. Moisander | M. Herrington | D. Hutton | N. Greer

47. ANTHRACNOSE ON STRAWBERRY IN FRANCE: SITUATION AND PERSPECTIVES

48. PATHOGENICITY OF UK ISOLATES OF COLLETOTRICHUM ACUTATUM AND RELATIVE RESISTANCE AMONG A RANGE OF STRAWBERRY CULTIVARS

D. Simpson | K. Hammond | S. Lesemann | A. Whitehouse

49. DEVELOPMENT OF A RAPID, RELIABLE SCREENING METHOD FOR PATHOGENICITY OF COLLETOTRICHUM SPECIES ON STRAWBERRY SEEDLINGS

S. Freeman | S. Horowitz | A. Zveibil | O. Yarden

50. SUCCESSFUL MANAGEMENT OF COLLETOTRICHUM CROWN AND STOLON ROT IN RUNNER PRODUCTION IN SUB-TROPICAL AUSTRALIA

D. Hutton

51. QUALITY AND ANTIOXIDANT ACTIVITY CHANGES DURING LOW-TEMPERATURE STORAGE OF STRAWBERRY FRUITS

C. Hansawasdi | S. Rithiudom | P. Chaiprasart

52. PRE-HARVEST CALCIUM EFFECTS ON SENSORY QUALITY AND CALCIUM MOBILITY IN STRAWBERRY FRUIT

J.L. Dunn | A.J. Able

53. THE EFFECT OF NITRATE: AMMONIUM RATIOS ON FRUIT QUALITY OF THE STRAWBERRY CULTIVAR ´SELVA´

T. Sadat Taghavi | H. Ebrahimzadeh | A. Rahemi | M. Babalar | A. Ebadi

54. THE EFFECT OF IRRIGATION METHOD ON THE QUALITY AND SHELF-LIFE OF STRAWBERRY FRUIT IN ORGANIC PRODUCTION

P. Parikka

55. EVALUATION OF COLD-STORE STRAWBERRY PLANT QUALITY ACCORDING TO DIFFERENT POSTHARVEST TREATMENTS

M. Gamardella | V. Diaz | P. Troncoso | I. Holmes | R. Pertuzé

56. QUALITY AND SHELF LIFE OF STRAWBERRY CULTIVARS IN POLAND

K.P. Rutkowski | D.E. Kruczynska | E. Zurawicz

57. FRESH AND PROCESSING QUALITY OF DIFFERENT STRAWBERRY CULTIVARS FOR LATVIA

V. Laugale | A. Bite

58. THE EFFECT OF CHITOSAN COATING AND CALCIUM CHLORIDE TREATMENT ON POSTHARVEST QUALITIES OF STRAWBERRY FRUIT (FRAGARIA × ANANASSA)

P. Chaiprasart | C. Hansawasdi | N. Pipattanawong

59. ANALYSIS OF VOLATILE AROMATICS IN ´TOYONOKA´ STRAWBERRIES (FRAGARIA × ANANASSA) USING PORAPAK Q COLUMN EXTRACTS

K. Fukuhara | X.X. Li | S. Yamashita | Y. Havata | Y. Osajima

60. ANALYTICAL AND SENSORY EVALUATION OF TWO STRAWBERRY CULTIVARS TO IMPROVE MARKET ACCEPTABILITY

A. Testoni | M. Nuzzi

61. EVALUATION OF POSTHARVEST QUALITY OF STRAWBERRIES IN ITALY

A. Testoni | F. Lovati | M. Nuzzi

62. STRAWBERRY FRUIT QUALITY: BIOCHEMICAL AND MOLECULAR ASPECTS

63. HIGH DENSITY, VERTICAL HYDROPONICS GROWING SYSTEM FOR STRAWBERRIES

G. Linsley-Noakes | L. Wilken | S. de Villiers

64. OUT-OF-SEASON STRAWBERRY PRODUCTION IN NORWAY: YIELD RESPONSES OF CV. KORONA TO PHOTOPERIOD PRECONDITIONING TREATMENT

A. Sønsteby | O.M. Heide | S. Grimsby | L. Grimsby

65. POSITIVE EFFECT OF SORGHUM GROWN AS A SOIL-IMPROVING CROP ON THE CO₂ ENVIRONMENT IN GREENHOUSES AND APPARENT PHOTOSYNTHESIS OF STRAWBERRY

Y. Yoshida | Y. Morimoto | K. Yokoyama | T. Ohnishi

66. CHILLING UNIT MODEL FOR GREENHOUSE PRODUCTION OF STRAWBERRY CV. ´ELSANTA´

P. Lieten

67. STRAWBERRY PRODUCTION IN SOILLESS SYSTEMS WITH SLOW SAND FILTRATION

J. Lopez-Medina | A. Peralbo | F. Flores

68. USING LATENT HEAT OF WATER EVAPORATION TO COOL THE CULTURE MEDIUM FOR HIGH-BENCH STRAWBERRY CULTURE

T. Ikeda | H. Kumakura | H. Hamamoto | T. Fujiwara | T. Shimazu

69. EFFECT OF K:CA:MG RATIO ON PERFORMANCE OF ´ELSANTA´ STRAWBERRIES GROWN ON PEAT

P. Lieten

70. EFFECT OF METOSULAM AND GLYPHOSATE ON FLOWER ABNORMALITIES OF STRAWBERRY

P. Lieten

71. EFFECTS OF SODIUM ON PERFORMANCE OF ´ELSANTA´ STRAWBERRIES GROWN ON PEAT

P. Lieten

72. STRAWBERRY QUALITY IN SOILLESS SYSTEMS

73. MALFORMATION OF STRAWBERRY FRUIT (FRAGARIA × ANANASSA) IN GLASSHOUSE PRODUCTION IN SPRING

G. van Kruistum | G. Blom | B. Meurs | B. Evenhuis

74. STRAWBERRY PRODUCTION IN SOUTH AMERICA

M. Gambardella | R. Pertuzé

75. ECONOMICS OF OUT-OF-SEASON STRAWBERRY PRODUCTION IN CROATIA

M. Njavro | B. Duralija

76. EVALUATION OF STRATEGIES TO COMMERCIALISE NEW STRAWBERRY CULTIVARS IN WESTERN AUSTRALIA

D.R. Phillips | D.G. Gatter

77. BUILDING BETTER INFORMATION PRODUCTS BY ASSESSING THE INFORMATION NEEDS OF GROWERS OF SUBTROPICAL STRAWBERRIES IN QUEENSLAND

N.T. Vock | G.N. Greer

78. STRAWBERRY BREEDING IN A SUBTROPICAL ENVIRONMENT

M.E. Herrington | C.K. Chandler

79. PROGRESS IN STRAWBERRY BREEDING IN ITALY

80. BREEDING ORNAMENTAL STRAWBERRIES

G. Bentvelsen | B. Bouw

81. RECENT PROGRESS IN STRAWBERRY BREEDING IN CHINA

Z. Yuntao | W. Guixia | D. Jing | L. Jiajun | D. Mingqin

82. USDA-ARS STRAWBERRY ANTHRACNOSE RESISTANCE BREEDING PROGRAM

B.J. Smith

83. THE DETECTION OF QTL ASSOCIATED WITH VEGETATIVE AND REPRODUCTIVE TRAITS IN DIPLOID FRAGARIA: A PRELIMINARY STUDY

D.J. Sargent | N.H. Battey | M.J. Wilkinson | D.W. Simpson

84. GENETIC ENGINEERING OF STRAWBERRY CV. FIREWORK AND CV. SELEKTA FOR TASTE IMPROVEMENT AND ENHANCED DISEASE RESISTANCE BY INTRODUCTION OF THAUII GENE

K.A. Schestibratov | S.V. Dolgov

85. PEDIGREE GENOTYPING: A NEW PEDIGREE-BASED APPROACH OF QTL IDENTIFICATION AND ALLELE MINING BY EXPLOITING BREEDING MATERIAL

86. DIFFERENTIAL EXPRESSED GENOME FRACTIONS AND TRANSCRIPTIONAL FACTOR DOMAIN ISOLATION IN FRAGARIA SPP.

L. Milella | M. Lapelosa | I. Greco | G. Martelli

87. CHARACTERIZATION OF STRAWBERRY GENOTYPES BY PTR-MS SPECTRAL FINGERPRINTING: A THREE YEAR STUDY

88. USEFULNESS OF SELECTED STRAWBERRY (FRAGARIA × ANANASSA) GENOTYPES FOR BREEDING LATE RIPENING CULTIVARS

E. Zurawicz | A. Masny | W. Madry

89. CROSS BREEDING WITH ACCESSIONS OF FRAGARIA CHILOENSIS RESULTING IN SELECTIONS WITH OUTSTANDING DISEASE RESISTANCE AND FRUIT QUALITY CHARACTERISTICS

K. Olbright | D. Ulrich | B. Dathe

90. TRANSGENE ENVIRONMENTAL RELATIONSHIP IN STRAWBERRY

91. DEVELOPMENT OF PCR-RFLP MARKER ON STRAWBERRY AND THE IDENTIFICATION OF CULTIVARS AND THEIR PROGENY

M. Kunihisa | N. Fukino | S. Matsumoto

92. ´AGUEDILLA´: A NEW STRAWBERRY CULTIVAR FROM THE SPANISH PUBLIC BREEDING PROGRAMME

93. STRAWBERRY GERMPLASM CONSERVATION: THE COLLECTION AT IFAPA-CIFA (MÁLAGA, SPAIN)

94. OPTIMIZATION OF A LIQUID MEDIUM FOR GERMINATION OF STRAWBERRY POLLEN

M. Ariza | J.M. Lopez-Aranda | C. Soria

95. FIRST EVALUATION OF STRAWBERRY GENOTYPES GROWN IN ITALIAN MOUNTAIN AREAS

96. ISOLATION AND CHARACTERIZATION OF GENES INVOLVED IN THE BIOSYNTHESIS AND SIGNALLING PATHWAY FOR ETHYLENE IN STRAWBERRY

A. Balogh | E. Kiss | T. Koncz | F. Dénes | L. Heszky

97. STRAWBERRY BREEDING FOR THE CENTRAL PLATEAU OF MÉXICO

98. THE STRAWBERRY BREEDING PROGRAM OF ´FRESAS NUEVOS MATERIALES S.A.´ (FNM): FIRST CULTIVARS DEVELOPED

A. Refoyo

99. ADVANCES IN STRAWBERRY BREEDING IN CHINA

M. Zhao | J. Su | Y. Qiang | Z. Wang

100. SELECTION OF NEW STRAWBERRY VARIETIES SUITABLE FOR PROCESSING – CULTIVAR EFFECTS ON THE QUALITY OF STRAWBERRY SORBET

A. Avitabile-Leva | A. Martinelli | M. Leiss | G.F. Castagnoli | A. Maestrelli

101. TWO NEW STRAWBERRY CULTIVARS FROM EAST MALLING RESEARCH

D. Simpson | K. Hammond | A. Whitehouse

102. THE APPLICATION AND SELECTION OF NEW STRAWBERRY VARIETIES OF SHIMEI SERIES

L. Yang | L. Li | L. Yang | B. Hao

103. QTL ANALYSIS FOR SUGARS AND ORGANIC ACIDS IN STRAWBERRY FRUITS

104. FLAVOUR CONTROL IN STRAWBERRY BREEDING BY SENSORY AND INSTRUMENTAL METHODS

D. Ulrich | E. Hoberg | K. Olbricht

105. GERMINATION CHARACTERISTICS OF TWEEZER-HARVESTED SEEDS IN STRAWBERRY CULTIVARS (FRAGARIA × ANANASSA DUCH.)

T. Yanagi | N. Okuda | T. Takamura

106. THE EFFECT OF DIFFERENT ORGANIC MULCHES ON STRAWBERRIES

V. Laugale | A. Bite | I. Morocko

107. TESTING OF STRAWBERRY CULTIVARS FOR ORGANIC PRODUCTION BASED ON DIFFERENT METHODS

108. DEVELOPMENT OF A COMMERCIALLY VIABLE SYSTEM FOR ORGANIC STRAWBERRY RUNNER PRODUCTION

M. Walter | C. Snelling | K.S.H. Boyd-Wilson | G. Williams | G.I. Langford